Microbiology Test

Trichomonas preparation

Dr. Nithin Jayan
Medically Reviewed by Dr. Nithin Jayan, MBBS, DNB
Last Updated on Apr 29, 2014
Email Print This Page Comment bookmark
Font : A-A+

Test : Trichomonas preparation

Indications : T vaginalis wet preparation is commonly used to diagnose T vaginalis infection.

To carry out wet mount microscopy, vaginal fluid is collected from the posterior fornix region of the vagina using a sterile cotton swab. It is placed in plastic tubes containing 0.5 ml of 0.9% saline and vigorously rotated to express as much of vaginal fluid as possible. One drop of this solution is placed on a micro slide and a cover slip placed over it. This is then examined at magnification of 200× within an hour of sample collection.

This test is only 60% sensitive but has been done profusely as it is simple and inexpensive.

Culturing the vaginal fluid sample is another method of diagnosis. Of late PCR method has replaced the wet mount preparation and culture methods as the golden standard for diagnosing T.vaginalis infections.

Physiology : Trichomonas vaginalis infections are caused by a flagellated parasite that specifically infests the urinary tract. The infection is very common in women, but highly curable.

In women, T vaginalis infections can range from being asymptomatic to acute inflammatory vaginitis. In an infected woman the ectocervix is inflamed and displays the classical ‘strawberry appearance’.

T vaginalis infections are also known to bring about pregnancy- related complications and increase the risk for HIV infection. In men, typically, it is asymptomatic but of late, its role in causing non-gonococcal urethritis has been recognized.

Normal Range : Negative, no trichomonads identified.

Interpretation : Normal - Negative

No Trichomonas is seen in the wet preparation indicating that the vaginal infection is not due to these flagellate parasites.

Abnormal - Positive

Motile Trichomonas is observed in the wet preparation indicating that these parasites are the causative agents of the vaginal infection. The number of motile organisms decreases with time and hence microscopy must be carried out as early as possible after collection.

Sample : vaginal, cervical, urethral swabs, prostatic fluid, urine sediment.

Test Method : Wet mount microscopic examination, pap smear.

Related Tests : cervical vaginal cytology, Genital culture



References : +



Revise This Test    Add New Test

Post a Comment

Comments should be on the topic and should not be abusive. The editorial team reserves the right to review and moderate the comments posted on the site.
Notify me when reply is posted
I agree to the terms and conditions

Lab Test A - Z

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Lab Test Search

Premium Membership Benefits

Medindia Newsletters

Subscribe to our Free Newsletters!

Terms & Conditions and Privacy Policy.

Stay Connected

  • Available on the Android Market
  • Available on the App Store