Highlights Antimicrobial resistant bacteria are becoming more common in pathogens causing pneumonia, diarrhea and sepsis. An inexpensive smartphone attachment can conduct automated antimicrobial susceptibility testing. Using smartphone, a team of UCLA researchers have developed an automated diagnostic test reader for antimicrobial resistance. The technology could lead to routine testing for antimicrobial susceptibility in areas with limited resources. Antimicrobial-resistant bacteria are posing a severe threat to global public health. In particular, they are becoming more common in bacterial pathogens responsible for high-mortality diseases such as pneumonia, diarrhea and sepsis. The UCLA researchers have developed a simple and inexpensive smartphone attachment that can conduct automated antimicrobial susceptibility testing. ‘The mobile reader could eliminate the need for trained diagnosticians to perform antimicrobial susceptibility testing and reduce the cost barrier for routine testing.’ "This work is extremely important and timely, given that drug-resistant bacteria are increasingly becoming a global threat rendering many of our first-line antibiotics ineffective," said Aydogan Ozcan, Chancellor's Professor of Electrical Engineering and Bioengineering at the UCLA Henry Samueli School of Engineering and Applied Science. "Our new smartphone-based technology can help put laboratory-quality testing into much wider adoption, especially in resource-limited regions." The UCLA device connects to a smartphone and has a plate that can hold up to 96 wells for testing. An array of LEDs illuminates the sample and then the phone's camera is used to sense small changes in light transmission of each well containing a different dose selected from a panel of antibiotics. Images are sent to a server to automatically perform antimicrobial susceptibility testing and the results are returned to the smartphone in about one minute. The researchers then tested the device in clinical settings at UCLA. They used special plates prepared with 17 different antibiotics targeting Klebsiella pneumoniae, a bacteria containing highly resistant antimicrobial profiles. During the clinical tests, they used 78 samples from patients. Their results showed that the mobile-phone-based reader meets the FDA-defined criteria for laboratory testing, with a detection accuracy of 98.2 percent. The lowest concentration of antibiotic that prevented the growth of bacteria is used to track drug resistance. A criterion -- that is susceptible to antibiotics or resistant to them -- is assigned to each bacteria/drug combination in order to guide the physician in treatment decisions. A susceptible result indicates that the organisms that have infected the patient should respond to therapy, while a resistant organism will not be inhibited by the concentrations of antibiotic achieved with normal dosages used for that drug. "This mobile reader could eliminate the need for trained diagnosticians to perform antimicrobial susceptibility testing, reduce the cost barrier for routine testing, and assist in tracking of bacterial resistance globally," Garner said. Di Carlo added, "An additional advantage of this technology is the possibility of examining bacterial growth in the presence of a drug at an earlier time point than is currently read, (about 24 hours). This could allow for a more rapid turnaround time of the results to the physician, which might help save lives." Reference Aydogan Ozcan et al., UCLA researchers combat antimicrobial resistance using smartphones, Scientific Reports (2016). Source: Medindia << Taste Might Not Be the Reason for Over Eating, Weight Gain Cytomegalovirus Linked to Childhood Acute Lymphocytic Leukem... >> Recommended Reading Antibiotics Antibiotics are among the most used and abused medications. This article explains some general features about antibiotics. READ MORE Natural Antibiotics to Fight Bacterial Infections Fighting infections the natural way and preventing them is always more effective than consuming medicines and antibiotics to treat them. READ MORE Drug Resistance - Antibiotic Resistance Drug resistance is often a problem in malaria, tuberculosis, HIV, sexually transmitted diseases and hospital acquired diseases. Judicious use of antibiotics can control the problem. READ MORE Interaction of Antibiotics with Dairy Products Antibiotics like tetracyclines and fluoroquinolones used in the treatment of bacterial infections interact mainly with dairy products and lead to adverse side effects. READ MORE Food Preservatives - How Safe Are They? Food preservatives are basically antimicrobials or anti-oxidants that help preserve food by destroying the microbes and stopping decomposition of food products. READ MORE Most Popular on Medindia Nutam (400mg) (Piracetam) Blood Donation - Recipients Drug Side Effects Calculator More News on: Food Preservatives - How Safe Are They?