Genetic change to histones - the non-DNA structures in the cell is enough to cause cancer, with no other cooperating DNA mutations, reveal findings published in Science .
Researchers have known for some time that histones play a role in switching genes on or off in cells by exposing or covering up various parts of the genome, for instance. But they are just beginning to learn about how changes to these non-DNA structures can affect development and health--a field known as epigenetics--and how they are regulated. Now, for the first time, they've shown that a change to the structure of a histone can trigger a tumor on its own.
A surprising source
To explore whether and how the mutation causes the tumor, the researchers inserted the H3 histone mutation into mouse mesenchymal progenitor cells (MPCs), which generate cartilage, bone and fat. They then watched these mutant cells lose the ability to differentiate in the lab. Next, they injected the mutant cells into living mice, and the animals developed the tumors rich in MPCs, known as an undifferentiated sarcoma.
"Although researchers knew that a certain type of tumor--chondroblastoma--carried a mutation in the H3 histone and no other mutation, this is the first study to show that the mutation indeed causes tumors, even without any other DNA mutation present," says Lu.
Next, the researchers tried to understand how the mutation causes the tumors to develop. They found that, when the mutation occurs, the cell becomes locked in a proliferative state--meaning it divides constantly, leading to tumors. Specifically, the mutation inhibits enzymes that normally tag the histone with chemical groups known as methyls, allowing genes to be expressed normally.
In response to this lack of modification, another part of the histone becomes over-modified, or tagged with too many methyl groups. "This leads to an overall resetting of the landscape of chromatin, the complex of DNA and its associated factors, including histones," says co-author Peter Lewis, a former postdoctoral fellow in the Allis lab and currently an assistant professor at the University of Wisconsin-Madison. This "resetting" is what locks the cell into its proliferative state.
A new target
The findings suggest that researchers should be on the hunt for more mutations in histones that might be driving tumors, says Lu. Meanwhile, he and his colleagues are trying to learn more about how this specific mutation in histone H3 causes tumors to develop. "For instance, we want to know which pathways cause the mesenchymal progenitor cells that carry the mutation to continue to divide, and not differentiate into the bone, fat, and cartilage cells they are destined to become," he says.
Once researchers understand more about these pathways, says Lewis, they can consider ways of blocking them with drugs, particularly in tumors such as MPC-rich sarcomas--which, unlike chondroblastoma, can be deadly. In fact, drugs that block the pathways may already exist and may even be in use for other types of cancers.
"One long-term goal of our collaborative team is to better understand fundamental mechanisms that drive these processes, with the hope of providing new therapeutic approaches," says Allis.