In order to map the 3-D model of GPCR, an important cellular gatekeeper, researchers have now used one of the brightest X-ray sources ever. The new technique is a major advance in exploring GPCRs, a vast, hard-to-study family of proteins that plays a key role in human health and is targeted by an estimated 40 percent of modern medicines.
The research, performed at the Linac Coherent Light Source (LCLS) X-ray laser at the Department of Energy's (DOE's) SLAC National Accelerator Laboratory, is also a leap forward for structural biology experiments at LCLS, which has opened up many new avenues for exploring the molecular world since its launch in 2009.
X-Ray Laser can Solve Protein Structures from Scratch
For the first time a study shows that X-ray lasers can be used to generate a complete 3-D model of a protein without any prior knowledge of its structure.
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"For the first time we have a room-temperature, high-resolution structure of one of the most difficult to study but medically important families of membrane proteins," said Vadim Cherezov, a pioneer in GPCR research at The Scripps Research Institute who led the experiment. "And we have validated this new method so that it can be confidently used for solving new structures."
In the experiment, published Dec. 20 in Science, researchers examined the human serotonin receptor, which plays a role in learning, mood and sleep and is the target of drugs that combat obesity, depression and migraines. The scientists prepared crystallized samples of the receptor in a fatty gel that mimics its environment in the cell. With a newly designed injection system, they streamed the gel into the path of the LCLS X-ray pulses, which hit the crystals and produced patterns used to reconstruct a high-resolution, 3-D model of the receptor.
Source: Eurekalert
Source: Eurekalert
Free X-Ray Services at Government Hospitals in Uttar Pradesh
Uttar Pradesh health minister Ahmad Hasan said the state government has decided to provide free X-ray facility at all government hospitals in the state.
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 Japanese Medics Use Mobile X-rays and Tablet Computers for Helping Victims of Philippines TyphoonWireless mobile X-ray kits that make use of tablet computers have been deployed by Japanese medics who are treating victims of the Philippines typhoon. | Advertisement
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