New nano-thermometer was found to take temperature inside cells. Rice University scientists used the light-emitting properties of particular molecules to create a fluorescent nano-thermometer.
The Rice lab of chemist Angel Martí revealed the technique in a Journal of Physical Chemistry B paper, describing how it modified a biocompatible molecular rotor known as boron dipyrromethene (BODIPY, for short) to reveal temperatures inside single cells.
Use of Nanotechnology in Healthcare
Nanotechnology provides several potential solutions for many life-threatening diseases. Learn more about the role of nanotechnology in healthcare
Advertisement
‘Nano-thermometer can take a single cell’s temperature. ’
Tweet it Now
The molecule is ideally suited to the task. Its fluorescence lasts only a little while inside the cell, and the duration depends heavily on changes in both temperature and the viscosity of its environment. But at high viscosity, the environment in typical cells, its fluorescence lifetime depends on temperature alone.
It means that at a specific temperature, the light turns off at a particular rate, and that can be seen with a fluorescence-lifetime imaging microscope.
![Quiz on Nanotechnology and Nanoparticles]( "Quiz on Nanotechnology and Nanoparticles")
Nanotechnology refers to techniques that use engineering at a very small scale, that is, use nano-sized objects. Take this quiz to know more about nanotechnology.
Martí said colleagues at Baylor College of Medicine challenged him to develop the technology. "Everybody knows old thermometers based on the expansion of mercury, and newer ones based on digital technology," he said.
"But using those would be like trying to measure the temperature of a person with a thermometer the size of the Empire State Building."
The technique depends on the rotor. Martí and Rice graduate student and lead author Meredith Ogle constrained the rotor to go back and forth, like the flywheel in a watch, rather than letting it rotate fully.
"It pretty much wobbles," Martí said.
"What we measure is how long the molecule stays in the excited state, which depends on how fast it wobbles," he said. "If you increase the temperature, it wobbles faster, and that shortens the time it stays excited."
The effect, Martí said, is conveniently independent of the concentration of BODIPY molecules in the cell and of photobleaching, the point at which the molecule's fluorescent capabilities are destroyed.
"If the environment is a bit more viscous, the molecule will rotate slower," Martí said. "That doesn't necessarily mean it's colder or hotter, just that the viscosity of the environment is different.
"We found out that if we constrain the rotation of this motor, then at high viscosities, the internal clock -- the lifetime of this molecule -- becomes completely independent of viscosity," he said. "This is not particularly common for these kind of probes."
Martí said the technique might be useful for quantifying the effects of tumor ablation therapy, where heat is used to destroy cancer cells, or in simply measuring for the presence of cancers. "They have a higher metabolism than other cells, which means they're likely to generate more heat," he said. "We'd like to know if we can identify cancer cells by the heat they produce and differentiate them from normal cells."
Source: Eurekalert
Quiz on Nanotechnology and Nanoparticles
Nanotechnology refers to techniques that use engineering at a very small scale, that is, use nano-sized objects. Take this quiz to know more about nanotechnology.
Advertisement
Martí said colleagues at Baylor College of Medicine challenged him to develop the technology. "Everybody knows old thermometers based on the expansion of mercury, and newer ones based on digital technology," he said.
"But using those would be like trying to measure the temperature of a person with a thermometer the size of the Empire State Building."
The technique depends on the rotor. Martí and Rice graduate student and lead author Meredith Ogle constrained the rotor to go back and forth, like the flywheel in a watch, rather than letting it rotate fully.
"It pretty much wobbles," Martí said.
"What we measure is how long the molecule stays in the excited state, which depends on how fast it wobbles," he said. "If you increase the temperature, it wobbles faster, and that shortens the time it stays excited."
The effect, Martí said, is conveniently independent of the concentration of BODIPY molecules in the cell and of photobleaching, the point at which the molecule's fluorescent capabilities are destroyed.
"If the environment is a bit more viscous, the molecule will rotate slower," Martí said. "That doesn't necessarily mean it's colder or hotter, just that the viscosity of the environment is different.
"We found out that if we constrain the rotation of this motor, then at high viscosities, the internal clock -- the lifetime of this molecule -- becomes completely independent of viscosity," he said. "This is not particularly common for these kind of probes."
Martí said the technique might be useful for quantifying the effects of tumor ablation therapy, where heat is used to destroy cancer cells, or in simply measuring for the presence of cancers. "They have a higher metabolism than other cells, which means they're likely to generate more heat," he said. "We'd like to know if we can identify cancer cells by the heat they produce and differentiate them from normal cells."
Source: Eurekalert
Smart Thermometer can Now Predict the Onset of an Outbreak
Real-time data from smart thermometers can effectively track and predict potential outbreak like influenza activity at national and regional levels.
Advertisement
 Ohio-Based Caring Things Introduces, Tympani, a New Smart ThermometerThe company has also developed an app for Android and iOS that displays and tracks temperature measurements from the device. | Advertisement
|
Advertisement
Latest Medical Gadgets
The Apple Watch monitors heart rate, VO2 Max (oxygen intake during exercise), and ECG, warning users globally of cardiac abnormalities.
Apart from monitoring basic health, the new apple watch series 9 features a new double tap gesture to control apps without touching the watch.
Our artificial olfactory system combines sensing and processing efficiently, mirroring the biological olfactory system's function for energy and space savings.
A portable, lightweight, and affordable exoskeleton designed for stroke patients promises expanded accessibility, particularly in areas with limited resources.
A textile-based wearable device that 'taps' a user's wrist with pressurized air, silently guides the person finding their way to their destination.
