Human microbiomes can be overwhelmingly variable due to differences
between people's environments, diets and genetics.
Numerous human diseases, including inflammatory bowel disease,
diabetes and autism spectrum disorders have been linked to abnormal gut
microbial communities, or microbiomes, but an open question is whether
these altered microbiomes are drivers of disease.
‘Inflammatory intestinal pathologies, such as Hirschsprung-associated enterocolitis or inflammatory bowel disease, can be explained as an overgrowth of certain pro-inflammatory groups of bacteria or a loss of anti-inflammatory bacteria.’
A new study at the University of Oregon, led by postdoctoral fellow
Annah Rolig, took aim at that question with experiments in zebrafish to
dissect whether changes in the abundance of certain gut bacteria can
cause intestinal inflammation.
The study, published in PLOS Biology
made use of a mutant zebrafish strain that models human Hirschsprung
disease, which is caused by loss of the gut neurons that coordinate gut
Just like Hirschsprung disease patients, who sometimes develop an
inflammatory condition called Hirschsprung-associated enterocolitis, a
subset of the fish developed intestinal inflammation.
The researchers successfully tracked how gut bacterial abundances
influenced inflammation. Fish with intestinal inflammation had a larger
abundance of a subset of bacteria that appeared to be pro-inflammatory,
which they confirmed by dosing the fish with one of these bacteria and
finding that it increased the severity of disease symptoms.
They also found a subset of bacteria that was depleted in the
inflamed intestines, but present in the mutant fish that remained
disease-free. Dosing the fish with a strain of these depleted bacteria
ameliorated the disease. Finally, they showed that they could cure the
inflammation by transplanting gut neurons from healthy fish into the
These studies demonstrate that inflammatory intestinal pathologies,
such as Hirschsprung-associated enterocolitis or inflammatory bowel
disease, can be explained as an overgrowth of certain pro-inflammatory
groups of bacteria or a loss of anti-inflammatory bacteria, said Judith
Eisen, a professor of biology and an expert on gut neurons in zebrafish.
The study stems from a long-term collaboration between Eisen and Karen Guillemin, who studies gut bacteria and inflammation.
"When we started this work, very few people were thinking about how
the nervous system and gut bacteria interact," said Eisen, who is a
member of the UO's Institute of Neuroscience. "Our studies demonstrate
how important it is to consider all the interacting cells of an organ,
including the microbial cells."
biologist and member of the UO's Institute of Molecular Biology, said, "The
zebrafish model allowed us to control those variables and see how
bacterial strains tracked with inflammation. From these patterns, we
could show that the drivers of disease can be a very few members of a
complex microbial community."
Identifying the bacteria that drive and protect against disease is
the first step toward developing microbial interventions and therapies,
said Rolig, a postdoctoral researcher in the UO's Institute of Molecular
"The fact that we could alleviate inflammation by adding back a
single key bacterial strain, suggests that it could be useful as a
probiotic for inflammatory diseases," said Rolig, who, along with Eisen,
is a scientist in the National Institutes of Health-funded Microbial
Ecology and Theory of Animals Center for Systems Biology, known as the
META Center, which Guillemin heads.
The next steps for the research group are to use what they have
learned from this zebrafish model of gut inflammation to design better
probiotics to treat intestinal inflammation.