Determination of cetuximab (CET) in human plasma samples can be made possible with highly sensitive and selective ELISA test.
Screening for HIV/AIDS Infection
Efficient screening and better drugs help reduce the prevalence of HIV infection. A number of tests like ELIZA & Western Blot are used for screening HIV.
Efficient screening and better drugs help reduce the prevalence of HIV infection. A number of tests like ELIZA & Western Blot are used for screening HIV.
‘A large number of CET samples can be processed with accuracy and precision using the ELISA test.’
The CET- EGFR complex formed onto the plate wells were quantified, for the first time, using alkaline phosphatase enzyme labeled anti-human IgG (ALP-IgG) and para-nitrophenyl phosphate substrate (pNPP) as a chromogenic substrate for alkaline phosphatase enzyme. The optimum conditions for conducting the proposed ELISA were established and its analytical performance was evaluated as per the guidelines for the validation of immunoassays for bioanalysis of therapeutic monoclonal antibody. The assay limit of detection was 0.0015 μg/mL and the effective working dynamic range was 0.005-6.25 μg/mL. The accuracy and precision of the assay were proved. The present assay with high throughput analysis very easy to perform in a 96-well plate and permits an operator to analyze a batch of ~ 200 samples, in triplicate. This facilitates the processing of a large number of samples in a clinical setting.
The developed method eliminates the need for pretreatment of plasma samples by affinity chromatography or other sophisticated equipment. ELISA for the CET is expected to contribute in studying its PK, PD and TDM. The used reagents are commercially available, and the color product can be easily measured by inexpensive equipment available in biological laboratories.
Portable Analyzer That Functions as Laboratory To Diagnose Diseases
The technology used with a smartphone is used to perform lab-grade medical diagnostic tests such as ELISA.
The technology used with a smartphone is used to perform lab-grade medical diagnostic tests such as ELISA.
Source-Eurekalert
Indian Doctors Urge Govt to Issue Advisory on ELISA Test
There is a need for an advisory from the Delhi government clarifying that Dengue ELISA is not vital or pre requisite for admission or therapy but haemogram is.
There is a need for an advisory from the Delhi government clarifying that Dengue ELISA is not vital or pre requisite for admission or therapy but haemogram is.
Novel Technique to Read ELISA Results
New technique to read ELISA results with the help of a cell phone camera has been revealed by researchers from Harvard Medical School and Harvard-MIT Health Sciences and Technology.
New technique to read ELISA results with the help of a cell phone camera has been revealed by researchers from Harvard Medical School and Harvard-MIT Health Sciences and Technology.
