Oregon State University (OSU) researchers have discovered a new approach to preserving tissues and even organs for later use. "This could be an important step toward the preservation of more complex tissues and structures," said Adam Higgins from OSU.
Ice-free cryopreservation has already found widespread use in simpler applications such as preserving semen, blood, embryos, plant seeds and some other biological applications. But it is often constrained by the crystallization that occurs when water freezes, which can damage or destroy tissues and cells, Higgins said.
To address this, researchers have used various types of cryoprotectants that help reduce cell damage during the freezing process -- among them is ethylene glycol, literally the same compound often used in automobile radiators to prevent freezing.
They found that if cells are initially exposed to a low concentration of cryoprotectant and time is allowed for the cells to swell, then the sample can be vitrified after rapidly adding a high concentration of cryoprotectants.
The end result is much less overall toxicity, Higgins said. The research showed that healthy cell survival following vitrification rose from about 10 percent with a conventional approach to more than 80 percent with the new optimized procedure.
The findings were published in the journal PLOS ONE.