Researchers led by Haiyang Dai from the Shantou University Medical College have made use of methanol-chloroform and perchloric acid in order to extract lipid-soluble metabolites and water-soluble metabolites from human mesenchymal stem cells.
Furthermore, a dual phase extraction method using methanol-chloroform and water was used to obtain both water and lipid fractions simultaneously. Among the different extraction procedures, perchloric acid was more efficient in extracting water-soluble metabolites and methanol-chloroform was efficient in extracting organic components compared with the dual phase extraction method. Nuclear magnetic resonance spectroscopy was found to be highly sensitive for analyzing lipid-soluble extracts. The researchers confirm that soluble metabolite spectrum has an important role to reflect lipid metabolic changes in the cytoplasm and cell membrane, a comprehensive analysis of spectral characteristics of water- and lipid-soluble metabolite extracts from mesenchymal stem cells provides a more comprehensive understanding of their metabolic characteristics, in order to achieve the best detection efficiency under the condition of reducing cell usage. These findings were published in the Neural Regeneration Research (Vol. 8, No. 22, 2013).