Currently it takes 24 hours for detection of infection in wounds. A new method could now enable physicians to detect infection in wounds in less than a minute, revealed a new research by the George Washington University's (GW) Victoria Shanmugam.
Shanmugam and co-authors Edgar Goluch, DiPietro Assistant Professor of Chemical Engineering at Northeastern University College of Engineering, and Agnes Chan, assistant professor at the J. Craig Venter Institute, published this new method in the Wound Repair and Regeneration. The method uses an electrochemical detection strategy to identify molecules produced by the bacteria Pseudomonas, which commonly infects chronic wounds.
The team tested the use of an inexpensive, disposable electrochemical sensor that immediately reveals bacteria based on the detection of pyocyanin, a bacterial quorum sensing molecule produced by Pseudomonas. The probe correctly identified the presence of the bacterium 71% of the time and correctly identified absence of the bacterium 57% of the time.
After further enhancement and testing, probes harnessing this methodology could potentially provide a way for physicians to detect wound infections at the bedside, allowing physicians to switch from broad-spectrum antibiotics to specific directed therapies sooner, lowering health care costs, minimizing drug resistance, and improving patient care outcomes.
Shanmugam said, "Infections are a major challenge in medicine, and by using this probe, we were able to harness one of the unique molecules produced by bacteria to detect infection. Through this ongoing collaboration with Dr. Goluch's team of engineers, we plan to continue to refine this testing method and hope to scale it up for detection of other bacteria and to optimize it for clinical use."