Micro Lab-on-a-Chip Device Helps Identify Blood Type in Minutes

A fully automated microchip has been developed, that can quickly and reliably determine a patient's blood type, without using time-consuming and specialized techniques. This device will prove useful under emergencies, where the patient requires an immediate blood transfusion.

The chip that contains a micro-sized "laboratory" with many compartments has been discovered, that can be used to identify blood types instantly. The blood samples will travel through the compartments in sequence and will be processed until results are obtained.

Blood transfusion, if performed promptly, is a potentially life-saving intervention for someone losing a lot of blood. However, blood comes in several types, some of which are incompatible with others. Transfusing an incompatible blood type can severely harm a patient. It is, therefore, critical for medical staff to know a patient's blood type before they perform a transfusion.

Blood Group Diet
What is right diet for you may be the wrong one for another. The diet that suits you will depend on your blood type. This diet is called the blood group diet.

‘The simple microchip device that reveals blood type within minutes will simplify medical care in emergencies and significantly reduce costs and the labor on parts of medical staff.’
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There are four major blood types--O, A, B, and AB. These types differ based on the presence or absence of structures called A antigens and B antigens on the surfaces of red blood cells. Blood can be further divided into positive and negative types based on the presence or absence of D antigens on red blood cells.

Medical professionals usually tell a patient's blood type with tests involving antibodies against the A and B antigens. When antibodies recognize the corresponding antigens, they bind to them, causing the blood cells to clump together and the blood to coagulate. Thus, specific antigen-antibody combinations tell us what the blood type of a blood sample is.

Yet, while the concept sounds straightforward, the equipment and techniques required are often very specialized. Tests, therefore, are non-portable, have high personnel cost, and can take over half an hour to yield results. This can prove problematic in several types of emergency situations.

Bombay Blood Group
Bombay blood group is a rare blood type in which the people have an H antigen deficiency. They can receive or donate blood only with other individuals with the same blood group.

Aiming to solve these problems, a team of scientists at Japan's Tokyo University of Science, led by Dr Ken Yamamoto and Dr Masahiro Motosuke, has developed a fully automated chip that can quickly and reliably determine a patient's blood type. In the words of Dr Motosuke, he and his colleagues "have developed a compact and rapid blood-typing chip which also dilutes whole blood automatically."

The chip contains a micro-sized "laboratory" with various compartments through which the blood sample travels in sequence and is processed until results are obtained. To start the process, a user simply inserts a small amount of blood, presses a button, and waits for the result. Inside the chip, the blood is first diluted with a saline solution and air bubbles are introduced to promote mixing. The diluted blood is transported to a homogenizer where further mixing, driven by more intensely moving bubbles, yields a uniform solution. Portions of the homogenized blood solution are introduced into four different detector chambers. Two chambers each contain reagents that can detect either A antigens or B antigens. A third chamber contains reagents that detect D antigens and a fourth chamber contains only saline solution, with no reagent, and serves as a negative control chamber in which the user should not observe any results. Antigen-antibody reaction will cause blood to coagulate, and by looking at which chambers have coagulated blood, the user can tell the blood type and whether the blood is positive or negative.

Blood Groups
The blood group of a person is determined by the presence or absence of certain inherited antigenic substances and certain acquired antibodies in blood.

Further, the user does not require specialized optical equipment to read the results. The design of the detector chambers allows the easy identification of coagulated blood with the naked eye. The device is also highly sensitive and can even detect weak coagulation.

During testing, the research team screened blood samples from 10 donors and obtained accurate results for all 10 samples. The time needed to determine a single sample's blood type was only five minutes.

Blood Transfusion - History, Procedure, Safety, Requirement
A blood transfusion is a medical procedure in which compatible, cross-matched blood (red blood cells) is transfused into a person through an intravenous line (IV).

Reflecting on the potential benefits of his team's invention, Dr Motosuke remarks, "The advancement of simple and quick blood test chip technologies will lead to the simplification of medical care in emergency situations and will greatly reduce costs and the necessary labor on parts of medical staff." Given the highly portable nature of the chip, Professor Motosuke also speculates that it could be used during aerial medical transport and in disaster response settings. This is a chip that has the potential to change the way emergency medical support is given.

Source-Eurekalert