Study Reveals How Gut Immune Cells are Kept in Control

Batteries of gut immune cells have a different composition that reduces their capacity of producing energy, keeping them in a controlled activated mode, revealed a study led by Marc Veldhoen, group leader at Instituto de Medicina Molecular João Lobo Antunes (iMM; Portugal). The study published in Immunology can give rise to new diagnostics and treatments for conditions affecting the digestive track such as gut inflammations or infections.

The outer layer of our bodies, the skin and intestine, contains a special population of white blood cells, called intraepithelial lymphocytes. It is largely unknown how the activity of these cells is controlled, not fully activated nor at rest. Using imaging and biochemical experiments, the research group led by Marc Veldhoen has now shown this is, at least in part, due to differences in the cells' "batteries" - the mitochondria. These energy-producing structures are present inside our cells regulate the cell's power. "We hypothesised that these gut-resident white blood cells may use energy in a different way. It was surprising to see that the detection of mitochondria gave a very different picture than seen in other white blood cells, forming the basis of a new hypothesis that the mitochondria themselves are different in these cells", explains Marc Veldhoen.

Using high magnification electron microscopy, the researchers observed that the mitochondria were present in abundance but seem to be different upon staining for light microscopy. Next, they studied the functionality of these batteries. "When we analysed in detail these structures, we found changes in the lipids that form a layer separating the mitochondria from the rest of the cell", says S?pela Konjar, joint first author of the study, adding that "these changes make the "batteries" work differently, as if they are in a "low energy mode".

When the lipid landscape was purposely altered, the researchers confirmed a change in the activation potential of the cells. "Our results showed that lipids in the mitochondria of these cells could alter their metabolic state and change their activity. When the mitochondrial lipids could not be arranged similar to those found in other white blood cells, the cells could not be properly activated when needed", explains Marc Veldhoen. The researcher further explains: "This knowledge allows us to investigate how we can inhibit these cells when they are too active and cause damage, such as in gut inflammations, or how we can activate them more in cases of gut infections.

Source-Eurekalert