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Embryonic Stem Cell Culturing Simplified by New Technology
"It's a technology that anyone can use. It's very simple," said Laura Kiessling, a UW-Madison professor of chemistry.
Scientists still use surfaces that contain mouse cells or mouse proteins to grow batches of human cells, whether embryonic or induced stem cells - which increases the chances of contamination by animal pathogens such as viruses, a serious concern for cells that might be used in therapy.
The new culture system utilizes a synthetic, chemically made substrate of protein fragments, peptides, which have an affinity for binding with stem cells.
It can culture cells in their undifferentiated states for up to three months and possibly longer and also works for induced pluripotent stem cells, the adult cells genetically reprogrammed to behave like embryonic stem cells.
Cells maintained in the system, Kiessling noted, were subsequently tested to see if they could differentiate into desired cell types, and performed just as well as cells grown in less defined, commercially available cell culture systems.
She added that the first clinical trials are underway and that as more tests in human patients are initiated, confidence in the safety of the cells will be paramount.
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More News on: Stem Cells - Cord Blood, Stem Cells - Fundamentals, Parkinsons Disease Surgical Treatment, Genetics and Stem Cells
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