About Careers Internship MedBlogs Contact us
Medindia LOGIN REGISTER
Advertisement

Researchers Reveal Polyclonality of BRAF Mutations in Acquired Melanocytic Nevi

by Rajashri on September 17, 2009 at 3:46 PM
Font : A-A+

 Researchers Reveal Polyclonality of BRAF Mutations in Acquired Melanocytic Nevi

A new brief communication published online September 14 in the Journal of the National Cancer Institute reveals that the polyclonality of BRAF mutations in melanocytic nevi suggests that mutation of BRAF may not be an initial event in melanocyte transformation.

To test the polyclonality of BRAF mutations, Minoru Takata, M.D., of the department of dermatology at the Shinshu University School of Medicine in Matsumoto, Japan, and colleagues separated approximately 50 single cells from each of 13 melanocytic nevi by using immunomagnetic beads or by laser-capture microdissection. They were then subjected to single-cell polymerase chain reaction and sequencing to determine BRAF mutations. In another experiment, BRAF and a neighboring single-nucleotide polymorphism were simultaneously amplified from nevi of four patients who were heterozygous for the polymorphism.

Advertisement

Although BRAF mutations were always found among nevus cells, cells that contained only wild-type BRAF predominated in nine of 13 nevi. When BRAF was sequenced from both alleles of four patients heterozygous for an adjacent polymorphism, both alleles harbored BRAF mutations.

"[P]olyclonality of BRAF mutations in the lesions of acquired melanocytic nevi suggests an alternative to the view that BRAF mutation is an initial event in melanocytic neoplasia," the authors write.



Source: Eurekalert
RAS
Advertisement

Advertisement
News A-Z
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
What's New on Medindia
Test Your Knowledge on Lung Transplantation
Baldness can be Cured and Prevented: let us see How!
Drinking Beer or Wine Every Day Could Cause Age-related Diseases
View all
Recommended Reading
News Archive
Date
Category
Advertisement
News Category

Medindia Newsletters Subscribe to our Free Newsletters!
Terms & Conditions and Privacy Policy.

More News on:
McArdle Disease How Do Viruses Mutate and Why Does it Matter? 

Most Popular on Medindia

Drug Side Effects Calculator Blood Donation - Recipients Calculate Ideal Weight for Infants Turmeric Powder - Health Benefits, Uses & Side Effects Indian Medical Journals Iron Intake Calculator Blood Pressure Calculator Nutam (400mg) (Piracetam) Noscaphene (Noscapine) Drug Interaction Checker
This site uses cookies to deliver our services. By using our site, you acknowledge that you have read and understand our Cookie Policy, Privacy Policy, and our Terms of Use