Protein-based drugs are commonly used for the treatment of various diseases like cancer, heart disease and rheumatoid arthritis. They are usually modified with chemical appendages to travel through the body or target specific tissues.
A recent study unveils new means of attaching lipid molecules to peptides for improving drug - delivery capabilities.
The new findings are reported in the Proceedings of the National Academy of Sciences.
Attaching lipids "improves all of these properties, enabling peptides to be more druglike," he said. Current methods for attaching lipids to peptides require the use of either harsh chemical solvents or expensive biological catalysts.
Nair and his colleagues focused on a little-known group of enzymes isolated from water-dwelling bacteria that have the remarkable ability to add lipids to a wide variety of proteins. The team performed a series of experiments on one family of these enzymes to discover how they recognize and interact with the peptides they modify.
The researchers discovered that one type of enzyme recognizes a simple, two-amino-acid sequence within its target proteins. They added this motif to two peptides selected at random and exposed the peptides to the enzyme. This caused the enzyme to add a lipid appendage to the proteins. The transformation was fast and efficient.
"Now that we have a very efficient way of attaching lipids to peptides, this opens up the possibility of using this approach to make large libraries of molecules that are more druglike than peptides," Nair said.