During the study, the researchers examined how strong memories are formed by creating new memories in the minds of mice while under sedation and monitoring their response to a memory-inducing stimulus afterwards, when they were awake.
"Our work is unique because it allows us to examine the cellular make-up of a memory, evaluate how the neurons change when a memory is formed and learn how that memory affects behavior," said Stephen Shea, Ph.D., the lead author of the study.
The researchers created memories by stimulating the release of noradrenaline - a chemical present in the body during strong emotional events ranging from excitement to fear.
Previous studies have established a link between noradrenaline and the formation of enduring memories, especially during intense social events such as mating and childbirth.
In mice and humans, the presence of noradrenaline also creates changes in the odor-processing center of the brain, called the olfactory bulb.
"When an animal forms a strong memory about another, it is reliant on odor cues and noradrenaline. Both need to be present at the same time in order for the memory to be formed. Long-term memories created under these conditions often result in a permanent change in behavior," Shea said.
The researchers administered anesthesia to a mouse and stimulated the release of noradrenaline with an electrode while wafting the scent of either food or the urine of another mouse under the nose.
"We wanted to see if these two elements - noradrenaline and odor - present at the same time were the key ingredients needed in the recipe for creation of memory - this is a concept that had not been directly tested before this study. In essence, we recreated the chemical reaction that would occur when the mouse experiences a social event, such as giving birth," Shea said.
Researchers knew they could observe brain activity in more detail when the mouse was under anesthesia. If awake, the mouse would be forming memories from the surrounding environment.
"When the animal is asleep, you can watch neurons in the brain rewire to store a memory and once awake see what the mouse learned even though it was asleep when the memory was created," Shea said.
Researchers saw an approximate 40 percent reduction in neuron activation after triggering the noradrenaline release - suggesting that a memory of the odor had been formed.
A day later, after the mouse was awake, the researchers observed changes in behavior in response to the scents, showing that they remembered the smells from when they were asleep.
"This work may have implications for furthering our understanding of how long-lasting memories are formed that are important to social bonding," said Richard Mooney, Ph.D., co-author and associate professor of neurobiology.
The study is published in the Journal of Neuroscience.