Vaccine candidate for SARS-CoV-2 developed at the University of Oxford is safe and induces immune reaction, raising hopes for a cure for COVID-19 that has killed tens of thousands and disrupted human activity across the globe, reports a new study.
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UK’s Vaccine Against COVID-19 is Safe, Induces Immune Reaction: The Lancet"
‘Promising early-stage results from a phase 1/2 clinical trial of the UK's vaccine candidate against SARS-CoV-2- the virus that causes COVID-19.
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An ideal vaccine against SARS-CoV-2 should be effective after one or two vaccinations, work in target populations including older adults and those with other health conditions, confer protection for a minimum of six months, and reduce onward transmission of the virus to contacts. The current trial is too preliminary to confirm whether the new vaccine meets these requirements, but phase 2 (in the UK only) and phase 3 trials to confirm whether it effectively protects against SARS-CoV-2 infection are happening in the UK, Brazil, and South Africa.
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Explaining how the vaccine works, study lead author Professor Andrew Pollard, University of Oxford, UK, says: "The new vaccine is a chimpanzee adenovirus viral vector (ChAdOx1) vaccine that expresses the SARS-CoV-2 spike protein. It uses a common cold virus (adenovirus) that infects chimpanzees, which has been weakened so that it can't cause any disease in humans, and is genetically modified to code for the spike protein of the human SARS-CoV-2 virus. This means that when the adenovirus enters vaccinated people's cells, it also delivers the spike protein genetic code. This causes these people's cells to produce the spike protein, and helps teach the immune system to recognize the SARS-CoV-2 virus."
He continues: "The immune system has two ways of finding and attacking pathogens - antibody and T cell responses. This vaccine is intended to induce both, so it can attack the virus when it's circulating in the body, as well as attacking infected cells. We hope this means the immune system will remember the virus so that our vaccine will protect people for an extended period. However, we need more research before we can confirm the vaccine effectively protects against SARS-CoV-2 infection, and for how long any protection lasts."
The new trial included 1,077 healthy adults aged 18-55 years with no history of COVID-19 and took place in five UK hospitals between 23 April and 21 May 2020 [2]. The data included in the paper covered the first 56 days of the trial and is ongoing.
The participants either received the new COVID-19 vaccine (543 people) or the meningococcal conjugate vaccine (534 people). One hundred thirteen participants (56 given the COVID vaccine, and 57 in the control group) were also asked to take paracetamol before and for 24 hours after their vaccination to help reduce vaccine-associated reactions (as the COVID-19 vaccine was given in a high dose to help induce a strong immune response).
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The participants were split into four groups. Group 1 (88 people) had additional safety monitoring to form the phase 1 part of the trial and had antibody and T cell responses assessed. Group 2 (412 people) had extra blood taken to assess for antibody and T cell responses, and group 4 (567 people) had serum taken to assess for antibody response only. In groups 1, 2, and 4, half, the participants received the COVID-19 vaccine, and half received the control vaccine. Group 3 (10 people) received only the COVID-19 vaccine, and were given an extra dose of vaccine 28 days after the first dose to determine the safety and whether this boosted antibody and T cell responses.
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Participants taking paracetamol around their vaccination had reduced pain, chills, feeling feverish, muscle ache, headache, and malaise in the two days following vaccination. In addition, in the ten people who received the extra dose of the COVID-19 vaccine, side effects were less common after the second dose.
The authors found that there were strong antibody and T cell responses from the vaccine. T cell responses targeting the SARS-CoV-2 spike protein were markedly increased (in the 43 participants studied), peaking 14 days after vaccination (median 856 spot-forming cells per million peripheral blood mononuclear cells), with this level declining slightly by day 56 of the trial (to median 424 spot-forming cells per million peripheral blood mononuclear cells). The T cell response did not increase with a second dose of the vaccine, which is consistent with other vaccines of this kind.
Antibody responses peaked by day 28 (median 157 ELISA units - studied in 127 participants) and remained high until the measurement at day 56 in the trial (median 119 ELISA units - studied in 43 participants) for those given a single vaccine. This response was boosted by a second dose (median 639 ELISA units at day 56 in these 10 participants).
28 days after vaccination, neutralizing antibody responses against SARS-CoV-2 were detected in 32 of 35 participants (91%) (when measured in MNA80 neutralization assay), and in 35 of 35 participants (100% - when measured in PRNT50 neutralization assay) who received a single dose of the COVID-19 vaccine. These responses were present in all participants who had a booster dose of the vaccine (nine of nine participants in MNA80 assay at day 42, and ten of ten in Marburg VN assay on day 56).
The authors found that taking paracetamol did not affect the immunogenicity of the COVID-19 vaccine.
Co-author, Professor Sarah Gilbert, University of Oxford, UK, says: "There is still much work to be done before we can confirm if our vaccine will help manage the COVID-19 pandemic, but these early results hold promise. As well as continuing to test our vaccine in phase 3 trials, we need to learn more about the virus - for example, we still do not know how strong an immune response we need to provoke to effectively protect against SARS-CoV-2 infection. If our vaccine is effective, it is a promising option as these types of vaccines can be manufactured on a large scale. A successful vaccine against SARS-CoV-2 could be used to prevent infection, disease, and death in the whole population, with high-risk populations such as hospital workers and older adults, prioritized to receive the vaccination."
The authors note some limitations, including that more research is needed to confirm their findings in different groups of people - including older age groups, those with other health conditions, and in ethnically and geographically diverse populations. The authors note that these groups are being recruited in their ongoing phase 2 and 3 trials of the vaccine in the UK, Brazil, and South Africa. In the current trial, 91% (979/1,077) of participants were white, and the average age of participants was 35 years.
They also note that a small number of participants had detectable neutralizing antibodies and T cell responses against SARS-CoV-2 spike protein before vaccination, likely to be due to past asymptomatic infection as potential participants with recent COVID-19-like symptoms or with a history of a positive PCR test for SARS-CoV-2 were excluded from the study.
The authors say the participants recruited in this study will be followed-up for at least one year to continue to study the vaccine's safety and the immune response it provokes.
Writing in a linked Comment discussing both Articles, lead author Assistant Professor Naor Bar-Zeev (who was not involved in the two studies), International Vaccine Access Center, Johns Hopkins Bloomberg School of Public Health, USA, says: "These trial reports are hugely anticipated. The results of both studies augur well for phase 3 trials, where the vaccines must be tested on much larger populations of participants to assess their efficacy and safety... Both trials used adenovirus vectors to deliver and study the COVID-19 vaccine, an innovative and efficient means of vaccine development in the midst of a pandemic. Capable of generating humoral, cellular, and innate responses, adenovirus vectored vaccines have much potential."
However, he warns of the preliminary nature of the two vaccine candidates. He continues: "The platform [adenovirus vectored vaccines] only achieved European Commission regulatory licensure on July 1, 2020, with the Ebola vaccine. Much remains unknown about these and other COVID-19 vaccines in development, including longevity of response and immunogenicity in older adults or other specific groups, such as those with comorbidities who are often excluded from clinical trials, or ethnic or racial groups more severely affected by COVID-19."
Source-Eurekalert