Medindia
Advertisement

New Electrochemical Assay Can Accurately Diagnose Cancer Causing Mutations from Blood

by Vishnuprasad on June 5, 2015 at 11:10 PM
Font : A-A+

New Electrochemical Assay Can Accurately Diagnose Cancer Causing Mutations from Blood

Cell free nucleic acids (cfNA) are genetic sequences. They are not attached to cells. They can be found circulating in the blood. These acids can be used to detect mutations in genetic sequences and can thereby be used as a 'liquid biopsy' tool for diagnosis of several types of cancer.

Scientists from University of Toronto have developed a simple method of analyzing the amount of mutated cfNA present in patient blood.

Advertisement

The study details the fabrication of an electrochemical clamp assay targeted towards detecting mutations in the KRAS gene, which causes lung, colorectal and ovarian cancer. The assay works by mixing in clamps that are specific for all other mutations except the mutation of interest, leading to a solution that has only the target mutation free of clamps.

This solution is then applied to a sensor, where the target mutation binds, and is detected with the help of an electrochemical sensor. The other mutations which have been clamped are not able to bind to the sensor and are finally washed away.
Advertisement

The electrochemical clamp assay requires much less volumes of serum, and can be performed on undiluted serum, which leads to simplicity in workflow and minimized sample loss. Also, the test can produce results in as little as 5 minutes, and can cut down on significant costs involved in cancer diagnosis.



Source: Medindia
Advertisement

Advertisement
News A-Z
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
News Category
What's New on Medindia
Gonorrhea
World Alzheimer's Day 2021 - 'Know Dementia, Know Alzheimer's
'Hybrid Immunity' may Help Elude COVID-19 Pandemic
View all

Medindia Newsletters Subscribe to our Free Newsletters!
Terms & Conditions and Privacy Policy.

More News on:
Thalassemia Cancer and Homeopathy Cancer Facts Cancer Tattoos A Body Art McArdle Disease Blood in Stools - Symptom Evaluation Bombay Blood Group Common Lifestyle Habits that Cause Diseases Health Benefits of Dandelion Plant 

Recommended Reading
Breast Cancer Screening using Mammogram
Breast cancer if detected early can be completely cured. Mammography is used to screen women for ......
Breast Cancer during Pregnancy
Management of breast cancer during pregnancy or pregnancy associated breast cancer requires special ...
Laryngeal Cancer
Laryngeal cancer is also called cancer of the larynx or throat cancer or laryngeal carcinoma. It ......
Maxillary Sinus Cancer
Cancers of paranasal sinuses are rare but if they arise, the most common sites affected are the ......
Blood in Stools - Symptom Evaluation
Blood in stools results from bleeding that arises from any part of the digestive tract. Causes of bl...
Bombay Blood Group
Bombay blood group is a rare blood type in which the people have an H antigen deficiency. They can r...
Common Lifestyle Habits that Cause Diseases
Cigarette smoking, unhealthy diets, overuse of alcohol, and physical inactivity are some of the most...
Health Benefits of Dandelion Plant
What is dandelion? Dandelion greens are nutrition powerhouses with a wide range of health benefits. ...
McArdle Disease
McArdle disease is a genetic disorder in which the body cannot breakdown glycogen in the muscles. It...
Tattoos A Body Art
Tattoos are a rage among college students who sport it for the ‘cool dude’ or ‘cool babe’ look...
Thalassemia
Thalassemia is an inherited blood disorder passed on through parental genes causing the body to prod...

Disclaimer - All information and content on this site are for information and educational purposes only. The information should not be used for either diagnosis or treatment or both for any health related problem or disease. Always seek the advice of a qualified physician for medical diagnosis and treatment. Full Disclaimer

© All Rights Reserved 1997 - 2021

This site uses cookies to deliver our services. By using our site, you acknowledge that you have read and understand our Cookie Policy, Privacy Policy, and our Terms of Use