Microbiology Test

Acid Fast stain

Dr. Simi Paknikar
Medically Reviewed by Dr. Simi Paknikar, MD
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Test : Acid Fast stain

Indications : The staining is used to detect the presence of mycobacteria, monitor therapy against mycobacterium, efective diagnosis of mycobacterial infection.

Physiology : Acid fast bacteria are surrounded by a waxy envelope that is resistant to destaining by acid alcohol. Heat, prolonged exposure peneterates the cell wall. The stained bacteria is resistant to decolorization with acid alcohol.

Normal Range : No acid fast bacteria. Positive smears are quantitated and reported as 1+ (3-9 bacteria in entire smear), 2+ (>10 in entire smear), or 3+ (1 or more bacilli per field) acid fast bacteria seen.

Sample : Fluid, pus, or abscess material

Test Method : Acid fast stain - Ziehl-Neelsen or fluorochrome stain

Related Tests : Mycobacteria by DNA probe, Skin Test, Tuberculosis.

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choky282, Nigeria

why do u heat at intervals during acid fast stain.

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