Last Updated on Oct 07, 2016

Diagnosis of Cholera

Cholera can be diagnosed with the help of detailed patient history and stool examination.

A patient history of watery stools especially during an epidemic should raise the suspicion of cholera.

Stool examination-

  • Macroscopic / Gross Examination-

Stools resembles ‘rice water’ and contain mucus

  • Microscopic Examination-

Dark field microscopic examination of stool sample shows rapidly motile V.cholerae bacteria. Epithelial cells are also present in the stool sample.

  • Culture-

A stool culture may be done to confirm the presence of V. cholerae. Enrichment is done in alkaline peptone water at pH 8.7 for 6-8 hours incubation.

Typical yellow color colonies appear in thiosulfate-citrate-bile salts-sucrose (TCBS) agar after 18 hours of incubation. Organisms can be confirmed from culture by staining and examining under microscope.

Specific Tests-

Vibrio cholerae are further identified by slide agglutination tests using anti-O group 1 antiserum and by biochemical reactions.

Comments

kelli.7 Wednesday, May 12, 2010

Grace is mean to me all the time!!!

Gavin Tuesday, November 18, 2008

In Zimbabwe the National Water Authority is failing to supply water to large parts of the urban areas. This has resulted in residences drawing on Boerhole Water and others purchasing from borehole owners. My question is what is the risk of being infected by the Cholera Bacteria especially in schools who use the water to wash with but not to drink?

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