1677 - Anton Von Leeuwenhoek visualized spermatozoa under the microscope.

1780 - Spallanzani’s experiment proved that physical contact between the male and female gamete is required for successful embryo development.

1899 - Russia works towards development of Artificial Insemination methods.

1922 - Reports of successful insemination experiments in horses published.

1939 - G. W. Salisbury pioneers research in animal breeding and artificial insemination.

1940 - Technical improvement in freezing and thawing of sperm preparations.

1950’s - Cornell suggests idea of adding anti-biotic to the sperm preparation media.

1953 - The first successful artificial insemination with frozen human semen was achieved.

1970, 1980’s - Enhancement in the sperm collection techniques.