New study reveals that in populations with a low tuberculosis (TB) incidence the majority of positives with the three tests commercially existing in the U.S for the TB diagnosis are false positives.
"We compared commercially available tests for latent tuberculosis infection (LTBI) in a diverse population with a low LTBI prevalence," said James Mancuso, MD, DrPH, of the Walter Reed Army Institute of Research Preventive Medicine Residency Program. "Our results suggest that in low-prevalence populations, most positive results obtained with these tests are false positives."
Tuberculosis
Tuberculosis, caused by Mycobacterium tuberculosis, primarily affects the lung. It may spread to other organs.
Advertisement
The findings were published online ahead of print publication in the American Thoracic Society's American Journal of Respiratory and Critical Care Medicine.
The cross-sectional study involved 2,017 military recruits at Fort Jackson, South Carolina, who completed a risk factor questionnaire and underwent testing with the 3 tests: 1) tuberculin skin test (TST), 2) the interferon gamma release assays (IGRAs) QuantiFERON®-TB Gold In-Tube test (QFT-GIT) and 3) the TSPOT® TB test (T-Spot). The Battey Skin Test (BST) was also administered to assess the impact of non-tuberculosis mycobacteria (NTM) reactivity on test discordance.
![Screening Tests for Tuberculosis]( "Screening Tests for Tuberculosis")
Tuberculin skin test and Interferon - Release Assays are tests used to screen for tuberculosis.
The specificities of TST, QFT-GIT, and T-Spot were not significantly different. Of 88 subjects with a positive test, 68 (77%) were positive to one test, 10 (11.4%) were positive to two tests, and only 10 (11.4%) were positive to all three tests. Bacille Calmette Guerin vaccination, tuberculosis prevalence in country of birth,and Battey skin test reaction size were associated with TST positive, IGRA negative test discordance, supporting evidence that NTM sensitization can cause false positive TST results. Greater quantitative test results and higher TB risk strata were associated with increased concordance between tests.
"Our data support a high proportion of false positives with any of these three tests in a low- prevalence population," added Dr. Mancuso, "as 77 percent of our subjects had positive results with only one test. Lower quantitative results were associated with a smaller risk for TB exposure and single positive tests, and lower risk for TB exposure was associated with decreasing test agreement."
There were some limitations to the study, including the lack of a gold standard for determining the presence of M. tuberculosis infection and administrative restrictions that resulted in an increased proportion of inadequate blood draws and TST reading times, which were slightly shorter than optimal.
"Low positive predictive value (PPV) is a well-known issue with the TST, and risk stratification is recommended to guide interpretation of the test," concluded Dr. Mancuso. "Our study suggests that risk stratification may also increase the PPV and reduce the number of false positives with the IGRAs. In accordance with the CDC's recommendation, people at minimal risk of TB infection should not be targeted for LTBI testing, regardless of which test is used."
Source: Eurekalert
Screening Tests for Tuberculosis
Tuberculin skin test and Interferon - Release Assays are tests used to screen for tuberculosis.
Advertisement
The specificities of TST, QFT-GIT, and T-Spot were not significantly different. Of 88 subjects with a positive test, 68 (77%) were positive to one test, 10 (11.4%) were positive to two tests, and only 10 (11.4%) were positive to all three tests. Bacille Calmette Guerin vaccination, tuberculosis prevalence in country of birth,and Battey skin test reaction size were associated with TST positive, IGRA negative test discordance, supporting evidence that NTM sensitization can cause false positive TST results. Greater quantitative test results and higher TB risk strata were associated with increased concordance between tests.
"Our data support a high proportion of false positives with any of these three tests in a low- prevalence population," added Dr. Mancuso, "as 77 percent of our subjects had positive results with only one test. Lower quantitative results were associated with a smaller risk for TB exposure and single positive tests, and lower risk for TB exposure was associated with decreasing test agreement."
There were some limitations to the study, including the lack of a gold standard for determining the presence of M. tuberculosis infection and administrative restrictions that resulted in an increased proportion of inadequate blood draws and TST reading times, which were slightly shorter than optimal.
"Low positive predictive value (PPV) is a well-known issue with the TST, and risk stratification is recommended to guide interpretation of the test," concluded Dr. Mancuso. "Our study suggests that risk stratification may also increase the PPV and reduce the number of false positives with the IGRAs. In accordance with the CDC's recommendation, people at minimal risk of TB infection should not be targeted for LTBI testing, regardless of which test is used."
Source: Eurekalert
Detecting Tuberculosis: No Microscopes, Just Rats
Researchers have found out that the Gambian pouched rats can smell the tuberculosis bacteria in a sputum sample.
Advertisement
Advertisement
|
Advertisement
Recommended Readings
Latest Tropical Disease News
Nipah is a zoonotic virus, meaning it can be transmitted from animals to humans. Fruit bats serve as the natural reservoirs for Nipah virus.
Nipah Virus (NiV) or Hendra virus is a deadly virus that spreads via contact with the saliva, urine, or excreta of fruit bats or pigs.
Nipah virus is mainly a zoonotic disease, transferring from animals to humans, and can also spread via contaminated food or contact with infected individuals.
Japanese Encephalitis in Assam, causing over 15 deaths during the monsoon season, officials are on high alert.
New study indicates that maternal dengue fever, regardless of severity, has a profound influence on birth outcomes.
