Two-Cell Mouse Embryos are Planning Their Future: Bioengineering Study

Mouse embryos are contemplating their cellular fates in the earliest stages after fertilization, when the embryo has only two to four cells, discovered bioengineers at the University of California, San Diego. This is a major breakthrough that could upend the scientific consensus about when embryonic cells begin differentiating into cell types. Their research, which used single-cell RNA sequencing to look at every gene in the mouse genome, was published recently in the journal Genome Research. In addition, this group published a paper on analysis of "time-course" single-cell data which is taken at precise stages of embryonic development in the journal of Proceedings of the National Academy of Sciences.

"Until recently, we haven't had the technology to look at cells this closely," said Sheng Zhong, a bioengineering professor at UC San Diego Jacobs School of Engineering, who led the research. "Using single-cell RNA-sequencing, we were able to measure every gene in the mouse genome at multiple stages of development to find differences in gene expression at precise stages."

In Vitro Fertilization (IVF)

Encyclopedia section of medindia gives a brief introduction to infertility and reproduction

The findings reveal cellular activity that could provide insight into where normal developmental processes break down, leading to early miscarriages and birth defects.

The researchers discovered that a handful of genes are clearly signaling to each other at the two-cell and four-cell stage, which happens within days after an egg has been fertilized by sperm and before the embryo has implanted into the uterus. Among the identified genes are several genes belonging to the WNT signaling pathway, well-known for their role in cell-cell communications.

Egg Donation

The term 'Egg Donation' is commonly used to refer to the contribution of her eggs by a woman to another, who is incapable of producing her own.

The prevailing view until now has been that mammalian embryos start differentiating into cell types after they have proliferated into large enough numbers to form subgroups. According to the co-authors Fernando Biase and Xiaoyi Cao, when the first cell fate decision is made is an open question. The first major task for an embryo is to decide which cells will begin forming the fetus, and which will form the placenta.

The research was funded by the National Institutes of Health (DP2OD007417) and the March of Dimes Foundation. The publications are "Cell fate inclination within 2-cell and 4-cell mouse embryos revealed by single-cell RNA sequencing", Genome Research, November 2014 and "Time-variant clustering model for understanding cell fate decisions", PNAS, November 2014.

Source: Eurekalert

IVF Births Have Higher Risk of Complications, Finds Study

Researchers in Australia claim that IVF babies are at an increased risk of complications like stillbirth, birth defects. However, it is too immature to blame IVF treatment alone at this point of time.

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