The bioelectronic "nose" can specifically detect a key decay compound at low levels, enables people to potentially take action before the stink spreads.
Strong odors are an indicator that food has gone bad, but there could soon be a new way to sniff foul smells earlier on. As reported in ACS Nano, the bioelectric nose can detect rotting food, as well as be used to help find victims of natural disasters or crimes.
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‘Nanodisc-based bioelectronic nose with olfactory receptor produced in escherichia coli can be used for the assessment of the death-associated odor, cadaverine.’
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When food begins to rot, the smell that we find repulsive comes from a compound known as cadaverine. That's also the substance responsible for the stench of rotting bodies, or cadavers -- hence the name. The compound is the result of a bacterial reaction involving lysine, which is an amino acid commonly found in various food products.
Detecting Smell With E.coli- Embedded Nanodisc
A previous study has shown that a receptor in zebrafish has an affinity for cadaverine. To make this receptor in the laboratory, scientists have turned to E. coli as a host cell because it can easily produce large quantities of proteins. But the production of this receptor in E. coli has been a challenge because it needs to be in a membrane.
![Smart Label Could Detect Food and Cosmetic Spoilage, Contamination]( "Smart Label Could Detect Food and Cosmetic Spoilage, Contamination")
A low-cost, portable, paper-based sensor incorporated into smart labels helps to detect food and cosmetic spoilage and contamination with easy-to-read results.
One way to do this is to make the protein in a bacterial cell and reconstitute it in nanodiscs, which are water friendly, membrane-like structures that the receptor can reside in. So, Seunghun Hong, Tai Hyun Park and colleagues wanted to see if they could put the receptor into nanodiscs to create a sensitive and specific detector for cadaverine.
The researchers successfully produced copies of the receptor in E. coli and assembled them into nanodiscs. The receptor-containing nanodiscs were then placed in a special orientation on a carbon nanotube transistor, completing the bioelectronic nose.
During testing with purified test compounds and real-world salmon and beef samples, the nose was selective and sensitive for cadaverine, even at low levels. Additionally, the researchers say the detector could someday prove useful in finding bodies, since the compound is also produced when a person dies.
Source: Eurekalert
A previous study has shown that a receptor in zebrafish has an affinity for cadaverine. To make this receptor in the laboratory, scientists have turned to E. coli as a host cell because it can easily produce large quantities of proteins. But the production of this receptor in E. coli has been a challenge because it needs to be in a membrane.
Smart Label Could Detect Food and Cosmetic Spoilage, Contamination
A low-cost, portable, paper-based sensor incorporated into smart labels helps to detect food and cosmetic spoilage and contamination with easy-to-read results.
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One way to do this is to make the protein in a bacterial cell and reconstitute it in nanodiscs, which are water friendly, membrane-like structures that the receptor can reside in. So, Seunghun Hong, Tai Hyun Park and colleagues wanted to see if they could put the receptor into nanodiscs to create a sensitive and specific detector for cadaverine.
The researchers successfully produced copies of the receptor in E. coli and assembled them into nanodiscs. The receptor-containing nanodiscs were then placed in a special orientation on a carbon nanotube transistor, completing the bioelectronic nose.
During testing with purified test compounds and real-world salmon and beef samples, the nose was selective and sensitive for cadaverine, even at low levels. Additionally, the researchers say the detector could someday prove useful in finding bodies, since the compound is also produced when a person dies.
Source: Eurekalert
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