Previous research has shown that transplanting neural stem/progenitor cells (NS/PCs) into the spinal cord promotes functional recovery after spinal cord injury (SCI).
However, which transplantation sites provide optimal benefit? This question was investigated by a Japanese research team and their findings will be published in a future issue of Cell Transplantation, but are currently freely available on-line as an unedited early e-pub at www.ingentaconnect.com/content/cog/ct/pre-prints/content-cog_09636897_ct1058iwai.
"It is critical to determine the optimal transplantation site for NS/PCs aimed at treating SCI," said Dr. Masaya Nakamura of the Department of Orthopedic Surgery at the Keio University School of Medicine. Previous work by the same research team revealed that NS/PCs injected into non-injury sites such as by intravenous or intrathecal administration did not engraft to the injury site in sufficient numbers, but instead were often "trapped" in the lungs and kidneys. They concluded that intralesional application might be the most effective and reliable method for transplanting NS/PCs. This study, also using laboratory mice with SCI, sought to determine how effective intralesional injection might be. NS/PCs were obtained from mice transgenic for Venus and luciferase fusion protein, which allowed the cells to be tracked by bioluminescence imaging (BLI) after transplantation.
"However, analysis showed that brain-derived neurotropic factor expression was higher in the RC segment than in the E segment." This result may explain why transplanted NS/PCs appeared to differentiate more readily into neurons than astrocytes in the RC group due to the impact of the enhanced expression of the brain-derived neurotrophic factor. "This may mean that the microenvironments of the E and RC sites are similarly able to support NS/PCs transplanted during the sub-acute phase of SCI," they concluded." This study provides evidence that the lesion microenvironment can support cell survival" said Dr. John Sladek, Cell Transplantation section editor and professor of neurology and pediatrics at the University of Colorado School of Medicine "The next step is to determine the factors that will impact favorably on the optimization of the cell transplantation site."