In patients with Parkinson's disease, the first transplantation of stem cells is almost within reach.
However, it remains a challenge for researchers to control stem cells accurately in the lab in order to achieve successful and functional stem cell therapies for patients.
"In our preclinical assessments of stem cell-derived dopamine neurons we noticed that the outcome in animal models varied dramatically, even though the cells were very similar at the time of transplantation. This has been frustrating and puzzling, and has significantly delayed the establishment of clinical cell production protocols", says Malin Parmar who led the study conducted at Lund University as part of the EU network NeuroStemcellRepair.
This new insight has enabled a streamlined differentiation process resulting in pure populations of dopamine neurons of high quality.
"We have identified a specific set of markers that correlate with high dopaminergic yield and graft function after transplantation in animal models of Parkinson's disease. Guided by this information, we have developed a better and more accurate methods for producing dopamine cells for clinical use in a reproducible way", says first author Agnete Kirkeby.
The new results, published in two back-to-back articles in the leading journal in the field, Cell Stem Cell, propel stem cell therapy for Parkinson's disease towards clinical application. The first transplants are expected to be only a few years away.