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Researcher Use Algae to Make Nanocellulose for Biofuels

by Bidita Debnath on Apr 10 2013 10:42 PM

 Researcher Use Algae to Make Nanocellulose for Biofuels
Behind a new project, scientists believed that they have reached an advanced stage in their effort to turn algae into solar-powered factories for producing the "wonder material" nanocellulose.
Researcher R. Malcolm Brown, Jr., Ph.D, said if they can complete the final steps, they would have accomplished one of the most important potential agricultural transformations ever.

"We will have plants that produce nanocellulose abundantly and inexpensively. It can become the raw material for sustainable production of biofuels and many other products. While producing nanocellulose, the algae will absorb carbon dioxide, the main greenhouse gas linked to global warming," he stated.

Brown, who has pioneered research in the field for more than 40 years, spoke at the First International Symposium on Nanocellulose, part of the ACS meeting.

In the 1800s, French scientist Louis Pasteur first discovered that vinegar-making bacteria make "a sort of moist skin, swollen, gelatinous and slippery" - a "skin" now known as bacterial nanocellulose. Nanocellulose made by bacteria has advantages, including ease of production and high purity that fostered the kind of scientific excitement reflected in the first international symposium on the topic, Brown pointed out.

Brown recalled that in 2001, a discovery by David Nobles, Ph.D., a member of the research team at the University of Texas at Austin, refocused their research on nanocellulose, but with a different microbe.

Nobles established that several kinds of blue-green algae, which are mainly photosynthetic bacteria much like the vinegar-making bacteria in basic structure; however, these blue-green algae, or cyanobacteria, as they are called, can produce nanocellulose. One of the largest problems with cyanobacterial nanocellulose is that it is not made in abundant amounts in nature.

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If it could be scaled up, Brown describes this as "one of the most important discoveries in plant biology."

Since the 1970s, Brown and colleagues began focusing on Acetobacter xylinum (A. xylinum), a bacterium that secretes nanocellulose directly into the culture medium, and using it as an ideal model for future research. Other members of the Acetobacter family find commercial uses in producing vinegar and other products. In the 1980s and 1990s, Brown's team sequenced the first nanocellulose genes from A. xylinum. They also pinpointed the genes involved in polymerizing nanocellulose (linking its molecules together into long chains) and in crystallizing (giving nanocellulose the final touches needed for it to remain stable and functional).

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But Brown also recognized drawbacks in using A. xylinum or other bacteria engineered with those genes to make commercial amounts of nanocellulose. Bacteria, for instance, would need a high-purity broth of food and other nutrients to grow in the huge industrial fermentation tanks that make everything from vinegar and yogurt to insulin and other medicines.

Those drawbacks shifted their focus on engineering the A. xylinum nanocellulose genes into Nobles' blue-green algae. Brown explained that algae have multiple advantages for producing nanocellulose. Cyanobacteria, for instance, make their own nutrients from sunlight and water, and remove carbon dioxide from the atmosphere while doing so. Cyanobacteria also have the potential to release nanocellulose into their surroundings, much like A. xylinum, making it easier to harvest.

In his report at the ACS meeting, Brown described how his team already has genetically engineered the cyanobacteria to produce one form of nanocellulose, the long-chain, or polymer, form of the material.

And they are moving ahead with the next step, engineering the cyanobacteria to synthesize a more complete form of nanocellulose, one that is a polymer with a crystalline architecture. He also said that operations are being scaled up, with research moving from laboratory-sized tests to larger outdoor facilities.

Their report on advances in getting those genes to produce fully functional nanocellulose was part of the 245th National Meeting and Exposition of the American Chemical Society (ACS), the world's largest scientific society, being held here this week.

Source-ANI


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