Lead researcher A.N.M. Nazmul-Hossaini will hold an oral presentation on a study titled Validation of Salivary-Biomarkers for Sjögren's Syndrome Detection in US Population

In the study, researchers collected unstimulated whole saliva samples from 30 well-characterized pSS patients, 30 patients with clinically-diagnosed rheumatoid arthritis (RA) and 30 healthy subjects. The saliva levels of 10 pSS mRNA markers were quantified by qRT-PCR. The levels of three salivary pSS protein markers (B2M, SSS and SSB) were quantified by Enzyme-Linked Immunosorbent Assay. Group differences of expression levels were tested by t-test. A logistic regression model was built and receiver operating characteristic (ROC) curves were constructed to determine the diagnostic capabilities of the respective salivary biomarkers.
At the conclusion of the study, a panel of 3 salivary mRNA biomarkers (B2M, GIP2 and MNDA) and 3 protein biomarkers (B2M, SSA and SSB) were validated in both European and US population cohorts, thus permitting the translational and clinical utilization of these biomarkers in a non-invasive manner for real-time cost effective clinical applications. Supported by NIH/NIDCR Grant: R01-DE017593.
Source-Eurekalert