Infections involving multi-drug resistant bacteria are a major concern. Colistin is used as the "last-resort antibiotic" for dreaded multidrug-resistant pathogens, especially in hospitals. However, gut bacteria that have become insensitive to colistin now exist - owing to the mobile resistance gene mcr-1. In early 2016, bacteria carrying this resistance gene were detected in Germany for the first time. Since then, there has been growing concern that it may develop into a "superbug" against which even emergency antibiotics are no longer effective.
‘A rapid genotypic resistance test for colistin is the only way with which mobile resistance can be differentiated from common resistance, because phenotypically they are the same.’
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The
risk of a further spread of this colistin resistance is high because it
takes place through so-called mobile genetic elements (plasmids) which
are transferred between different types of bacteria relatively easily. "Confirming the mobile mcr-1 resistance gene as rapidly as possible is important, so as to prevent its further spread," emphasizes Linda Falgenhauer, DZIF scientist at the Justus Liebig University Giessen and one of the first authors of this study.
Together with her colleagues from Giessen University and scientists from the research association RESET, she tested a rapid genotypic resistance test for colistin that is already commercially available.
"This is the only way with which mobile resistance can be differentiated from common resistance, because phenotypically they are the same," explains Can Imirzalioglu, who is also first author of the study and DZIF scientist at the Giessen University.
Getting results rapidly
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The test could clearly differentiate between common colistin resistance and mobile resistance located on plasmids. "The test results become available in only twenty minutes," explains Judith Schmiedel from the Giessen Team. "With the conventional procedure, it takes several hours to get results.
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Source-Eurekalert