The nutritional preferences and growth characteristics of 96 diverse gut bacterial strains have been identified by a research team who successfully grew gut bacteria in the lab.
The bacteria living in the gut have a big impact on our health. But researchers still don't know what kind of food most of our gut bacteria like to live on, or precisely how they metabolize nutrients. The current paper reports on the growth characteristics of the main human gut bacteria in nineteen different growth media with well-defined recipes. Peer Bork, Kiran Patil and Nassos Typas, all group leaders at EMBL Heidelberg, led the work.
"Our resource provides scientists with tools to experimentally investigate the gut microbiome ecology, going beyond correlations and identifying causes and effects," says Nassos Typas.
"We were surprised to find new bacteria with the capability to utilize mucin, the protein that makes up mucus," says Kiran Patil. "These bacteria can contribute to inflammation and infection by weakening the protective mucus barrier lining the gut. Another surprise came from bacteria that proved to be inhibited by amino acids and short-chain fatty acids, common ingredients in most growth media. It turns out that rich media with many nutrients can be toxic for these species, whereas we used to think: the more food, the better."
Furthermore, even closely related bacteria sometimes had completely different nutritional preferences. This shows that microbiologists can't base their assumptions about metabolic capabilities on bacteria's genetic relationships alone.
The new scientific 'cookbook' is filled with molecular recipes on how to grow gut bacteria, providing the community with the tools for studying the structure and function of the human gut microbiome.