More than 200 ninjas gathered at the Tokyo gymnasium to fuel the two-day training seminar, designed particularly for women.
The disciples of Grandmaster Masaaki Hatsumi, the founder of the Bujinkan school, gathered from more than 17 countries at the Tokyo Budo-kan martial arts gymnasium to sharpen their self-defence skills.
Gone are the days when the Japanese historical masked agents carried out espionage acts against opposing warlords, they said.
The historical image of a ninja may attract many people, but they soon find the importance of the culture and philosophy behind it, said Sheila Haddad, an American instructor who helped organise the event.
"It's appealing to women because it does not rely on power and strength," she said, adding that many disciples are involved in law enforcement or the military in their countries.
Ninjutsu techniques as well as the ninja philosophy can be applied to real combat settings, said Doreen Montesclaros, 25, a soldier in the New Zealand army.
"Military martial arts as well as other (forms of martial arts) are very rigid and all about strength and all about being fitter and being stronger... overwhelming your opponents," she said.
But ninja ways are more relaxed, she said.
"Being surrounded by male colleagues, I find that it adds new dimensions to the way I approach battle scenarios. I come into it with an overall picture, as opposed to being stuck in the moment and relying on aggression," she said.
"It helps me realise the greatest weapon that I have. That's my mind and being able to see all the factors that make up situations."
Jinichi Kawakami, the 21st head of the Ban clan, a different line of ninjas that can trace its history back some 500 years, is considered by some to be the last living ninja and has said that "ninjas proper" no longer exist.