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New Virtual Staining Technique Using Infrared Spectroscopic Imaging Speeds Up Pathology

by Vishnuprasad on May 15, 2015 at 1:02 AM
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New Virtual Staining Technique Using Infrared Spectroscopic Imaging Speeds Up Pathology

Staining of cells in pathology allows clinicians and researchers to identify different cell types and to track their activity. It can be a slow and laborious process, which can also cause disruption to the sample being analyzed.

University of Illinois at Urbana-Champaign scientists have developed a virtual staining technique that doesn't require any actual staining while producing similar results.

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The technique uses infrared spectroscopic imaging to analyze the chemical composition of cells directly. The investigators were able to identify correlations that a computer can use to do quick pathology analysis by noting the spectral characteristics of light bouncing off the cells.

The scientists hope that the new method will drastically increase the speed of digital pathology studies, since manual aspects of staining and preparation may be avoided altogether. The technology relies on computation, instead of staining to provide images.
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"The imaging allows histology digital imaging without destroying tissue properties caused by staining, therefore the same slide can be used for other purposes. For research applications, it also allows higher throughput by rapidly marking up the tissues for regions of interest," said David Mayerich, the lead author of the study.



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