Have you ever wondered what microbes actually eat? Well even the researchers from North Carolina State University and the University of Calgary thought so too, and so they have devised a new way to determine what food do microbes eat. The findings of this study are published in the Proceedings of the National Academy of Sciences.
The researchers use a mass spectrometer to measure with very high accuracy the mass of molecules derived from the microbes in a community. Then they use a newly developed software program that allows them to link microbes with their substrates.
The basis for connecting microbe and substrate are so-called carbon stable isotope ratios - the ratios between naturally occurring forms of carbon with different masses. Nature contains both carbon-12, the most abundant form, and carbon-13, which has one more neutron than carbon-12. Each material has a very specific ratio of these two isotopes, which essentially can be used as the fingerprint or signature of the material. The new algorithm links the carbon isotope ratios of the substrates that are available to microbes in a given environment to the ratios found in the microbes themselves.
"Our method is based on the concept that you are what you eat," Kleiner said. "If there is a food source with a specific isotopic signature and we find a microbe with that same specific isotopic signature, we can make the connection between the two. A similar technique is actually used by archeological anthropologists, who can determine the type of diet a person ate by analyzing isotope ratios from hair or bone fragments."
Human hair is crucial for the technique to work. Kleiner surprised his hairdresser by bagging his hair after a haircut.
"We have to correct for a certain error occurring during mass spectrometry by using a calibration standard. As it turns out, a human hair is an excellent material to calibrate our instrument," Kleiner said.
To test the new algorithm, the researchers took 20 pure culture samples and showed that the software provided measurements consistent with mass spectrometry standards. The researchers also put together complex samples with different microorganisms and tested for individual signatures from different species within the complex community. Finally, the researchers studied a gutless marine worm that hosts a number of bacteria and used the software to test the metabolism of this symbiotic relationship.
Kleiner added that all the data from the study is publicly available, so other researchers can test and utilize the new software.