Study Provides Clues to Design Novel Cancer Therapies

Researchers analyzed metastatic tumor cells (MTCs) and identified eight functional processes expressed by MTCs across seven types of metastatic brain cancer. Two functional archetypes of metastatic cells were identified across seven different types of brain tumors, each containing both immune and non-immune cell types, as per the study published in CELL. Researchers found these specialized and complementary processes work together within single cells to shape two recurrent cell archetypes, one inflammatory and other proliferative, that co-exist within each metastatic tumor and are both shaped by immune cells.

Brain metastasis is the most common form of brain cancer, occurring nearly ten times more often than cancer which begins in the brain. While treatment options for brain metastases have improved in recent years, there is still much left to understand about metastasis formation.

The team combined high-dimensional single-cell analyses of human brain tissue metastases from the different cancer types and experimental models to identify and understand the recurrent patterns that characterize the process of metastasis formation in patients.

They also identified a comparable metastatic niche or microenvironment and an immunosuppressive stroma enriched with T-cells and metastasis-associated macrophages that seem to play a role in the dynamics of the two archetypes.

“These archetypes co-exist within each metastatic tumor,” said Gonzalez. “For the MTCs that are not proliferating, these cells get reprogrammed to express genes for inflammation, stress, and other changing conditions. These tumor-immune interactions are likely shaping the state of the MTCs.”

“Zena Werb was the first person that saw the potential and feasibility of collecting human metastases and combining them with cutting-edge technologies such as single-cell transcriptomics and CyTOF,” said Gonzalez.

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“She believed that by analyzing human brain metastases, we could determine the relationship between these cellular processes orchestrated by MTCs and their specific microenvironments.”

Werb, a world-renowned researcher in cancer biology and associate director for basic science at the UCSF Helen Diller Family Comprehensive Cancer Center, transformed the field by highlighting the critical role of cells’ local “neighborhoods” in determining tumor growth and behavior.

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Over four decades; her work laid the groundwork for the rise of immunotherapy and other modern approaches to cancer treatment. Werb passed away in 2020 at the age of 75, but her legacy lives on with her colleagues, for whom she was a mentor.

“From the beginning, she believed in this project and encouraged me to persevere even when the collection and processing of these rare and small samples were quite difficult,” said Gonzalez.

“Zena also helped orchestrate fruitful collaborations with UCSF colleagues Joanna Phillips, MD, PhD, and Matthew Spitzer, PhD, who were critical for this large project.”

Roose added that Gonzalez’s work provided an important foundation for the team’s collaborative efforts with the UCSF Endeavor program to better understand how metastases arise when cancer cells interact with host cells surrounding the tumor.

Roose has found it extremely rewarding to see Hugo through the home stretch of this brain metastasis project. “I can just see Zena walk into my office, giving us a thumbs up and a big hug,” Roose said.

Source-Medindia