Medindia
Advertisement

New Fluorescent Dye May Help Early Detection of Neurodegenerative Diseases

by Adeline Dorcas on November 30, 2018 at 3:09 PM
Font : A-A+

New Fluorescent Dye May Help Early Detection of Neurodegenerative Diseases

New fluorescent dye may help detect signs of neurodegenerative diseases earlier, reports a new study. The findings of the study are published in the Journal of Luminescence.

Signs of neurodegenerative diseases, visible years before the emergence of clinical manifestations, can be detected during the examination of medical samples by means of fluorescence microscopy. This is, however, on condition that suitably sensitive and selective dyes are used that bind to specific amyloid structures. The new dye, proposed by the Polish-American group of scientists, is a step towards the personalized neuro-medical prophylaxis of the future.

Advertisement


From memory disorders to the notorious Creutzfeldt-Jakob disease or Alzheimer's disease - dozens of diseases are related to the formation of amyloid fibrils deposits of complex shapes. They are deposited as a result of proteins misfolding, to structures mainly known as amyloid beta. There are grounds to believe that soon the first signs of neurodegeneration will be identifiable in medical laboratories at an earlier stage of development of brain disease, and in a much more precise way than ever before.

The new avenues of detection are being opened thanks to the achievements of a Polish-American team, which includes scientists from the Institute of Physical Chemistry of the Polish Academy of Sciences (IPC PAS) in Warsaw, Wroclaw University of Technology, the University of Michigan and the University of California, Santa Barbara.
Advertisement

With the aging of society, the importance of early detection of neurodegenerative diseases, usually manifesting in people at a later age, is growing. The stakes are high - just from looking at the data on Alzheimer's disease. While just 3% of people over 70 years of age suffer from the disease, as many as half of the population suffers after the age of 90, it is known, however, that this disease probably begins even 20 years before the onset of its first symptoms. In this situation, the early and precise detection of its harbingers takes on a particular significance.

"Before a lab technician examines a sample of a patient's cerebrospinal fluid under a fluorescence microscope, he must somehow label the required chemicals with a fluorescent dye. Usually, small molecules are used for this purpose, selected so that they only bind with the molecules that are to be detected. We have proved that we can successfully use the polythiophene derivative PTEBS as the dye. This is a polymer, that is, quite a big atomic structure. In practice, it has turned out that the molecule size of PTEBS is not only not a disadvantage, but in fact quite a significant advantage," says Dr. Piotr Hanczyc (IPC PAS, currently Faculty of Physics, University of Warsaw), the first author of the publication in the Journal of Luminescence.

The team's research has made it possible to conclude that the presence of both the required chemical molecules as well as their aggregates can be recorded using the PTEBS dye, even when they are present in the sample at significantly lower concentrations than detected by thioflavin T, currently one of the most popular fluorescent dyes used for labelling protein aggregates.

An important advantage of the new dye is related to the existence of polymorphic forms of amyloid, i.e., the fact that while one configuration of atoms in a molecule may be responsible for triggering neurodegenerative processes, the another can turn out to be harmless.

"Standard dyes are molecules of a small size. You cannot do too much with them, in addition, they have been thoroughly tested, and a lot is already known about them. The molecules of our dye are large, and many substituent groups are attached to the main chain. These groups can be modified and extended to a large extent, increasing the affinity of the dye not only to the selected form of amyloid but also to its specific polymorph. This gives us a lot to work with," emphasizes Dr. Hanczyc.

The new dye should help, among others, in the more precise determination of the polymorphic variations responsible for the course of neurodegenerative processes in patients. However, its applications in prophylactic examinations are particularly promising. They would allow for the selection of more effective treatment strategies, personalized for a particular patient. This sort of procedure would mean that it would be possible to significantly delay the development of neurodegenerative diseases and possibly even completely prevent them in the future.

Source: Eurekalert
Advertisement

Advertisement
News A-Z
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
News Category
What's New on Medindia
Gonorrhea
World Alzheimer's Day 2021 - 'Know Dementia, Know Alzheimer's
'Hybrid Immunity' may Help Elude COVID-19 Pandemic
View all

Medindia Newsletters Subscribe to our Free Newsletters!
Terms & Conditions and Privacy Policy.

More News on:
Spinocerebellar Ataxia 

Recommended Reading
New Antibody Test to Detect Proteins Implicated in Neurodegenerative Conditions
The antibody that binds to the tau-proteins, acts like a magnifying glass, amplifying tau levels so ...
Alzheimers Disease
Alzheimer's disease is a progressive neurodegenerative disease affecting memory and thinking and ......
Novel Finding Offers New Treatment Approach for Neurodegenerative Diseases
New findings connect ataxia or amyotrophic lateral sclerosis (ALS) to cellular stress which may ......
Multiple Targets in One Therapy for Neurodegenerative Diseases
Targeting multiple proteins known as "proteinopathies" at once may be the real key in treating ......
Spinocerebellar Ataxia
Spinocerebellar ataxia (SCA) is a progressive, neurodegenerative, genetic disease, which has no cur...

Disclaimer - All information and content on this site are for information and educational purposes only. The information should not be used for either diagnosis or treatment or both for any health related problem or disease. Always seek the advice of a qualified physician for medical diagnosis and treatment. Full Disclaimer

© All Rights Reserved 1997 - 2021

This site uses cookies to deliver our services. By using our site, you acknowledge that you have read and understand our Cookie Policy, Privacy Policy, and our Terms of Use