Scientists have revealed that by blocking signals from a key molecular receptor that normally switches on the intestine’s immune response may help reverse necrotizing enterocolitis (NEC), a leading cause of death in premature newborns.
The study was carried out under David J. Hackam and his laboratory team at the Children’s Hospital of Pittsburgh.They documented in their report that neonatal mice with inactivating mutations in the Toll-like receptor 4 (TLR4) are protected from NEC. “Toll-like receptors” are key players in the innate immune system. TLR4 receptors, protruding from enterocytes that form the innermost barrier-like layer of the small and large intestines, are primed to recognize pathogenic bacteria and sound the alarm.
However the researchers found that the stresses of oxygen deprivation and bombardment by bacterial toxins, conditions that can occur in premature infants with underdeveloped lungs, lead to excessive production of TLR4.
Just like an unstoppable alarm, the increased numbers of TLR4 blare out signals that eventually tip the cells into cellular suicide. They also stop enterocytes from migrating to close wounds in the intestines.
Such events, which do not occur in the TLR4-deficient mice, allow NEC to spread as the fragile lining of the gut gives way, releasing a flood of pathogens into the bloodstream.
Even with modern-day progresses in neonatal care, NEC affects 20 percent of premature babies and is fatal in nearly half of all cases, according to Hackam.
Advertisement
The TLR4 siren was silenced by shutting down FAK with small interfering RNA. Under these conditions, the researchers watched enterocytes regain the ability to migrate, a property important for healing the damaged tissue.
Advertisement
The study was presented at the American Society for Cell Biology 47th annual meeting.
Source-ANI
LIN/M
