According to a source, the American singer/actress who is known for her toned body, and is said to follow a strict routine of Yoga, ate nothing during the seven-hour flight and took vitamin shots before the landing.
"Seven hours is a long time to go without eating. She was also very quiet and only spoke to the air staff to ask for water," News.com.au quoted the source, as telling Metro.co.uk.
"Just before landing she brought out the phials of vitamins and injected herself," the source said.
Madonna's spokeswoman refused to comment on whether the star uses vitamin jabs.
Other celebrities who are rumoured to use the energy boosting shots include Lindsay Lohan and Britney Spears.
British Nutrition Foundation scientists Anna Denny said vitamin jabs could be useful for the elderly and very young.