Akie visited the Kalawati Saran Children's Hospital and interacted with children suffering from AIDS and their parents.She also met the hospital staff and doctors.
Earlier this morning, Akie visited a Japanese school here, as her husband talked business with the Indian leadership.
Mrs. Abe, who interacted with school children, said she hoped the two countries would be able build on their 50-year-old relationship.
"Japan and India have fostered good relations over the past 50 years. I was only hoping that the long and good relations are further promoted. Japan is important for India and India is important for Japan," she said.
Abe also joined a school children playing session, skipping ropes and building blocks with them.
Students dressed in Indian outfits danced to traditional music as part of a cultural show put up to welcome Japan's First Lady.
The school has around 160 Japanese students studying from kindergarten to Grade Nine.
Akie Abe is accompanying her husband Shinzo Abe on his visit to the country, which is aimed at improving strategic relations between Tokyo and New Delhi, and to boost trade with India.