Is Hydrolyzed Collagen Suitable for Nerve Cell Culture?

Dr. Mohsen Fathi Najafi and colleagues from Mashhad University of Medical Science, Iran, investigated the effects of hydrolyzed and non-hydrolyzed collagen and two-dimensional and three-dimensional collagen matrices on cell survival, attachment and neurite outgrowth of primary cultured nerve cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide co-lorimetric assay and inverted microscopy.

Hydrolyzed collagen facilitated nerve cell survival and neurite outgrowth, but it had no obvious influences on cell attachment. In contrast, non-hydrolyzed two-dimensional collagen matrix had no obvious effects on neurite outgrowth.

These findings, published the Neural Regeneration Research (Vol. 8, No. 23, 2013), suggest that hydrolyzed collagen is an ideal nerve cell culture media.

Source: Eurekalert