Bacterial skin infections caused by Staphylococcus aureus (staph) account for 14 million outpatient visits, nearly 500,000 hospital admissions and $3 billion to $4 billion in inpatient health care costs in the U.S. per year. New research conducted by researchers at Johns Hopkins, the University of California, Davis, and the National Institute of Allergy and Infectious Diseases and published in The Journal of Clinical Investigation finds the immune system might protect a person from recurrent bacterial skin infections caused by Staphylococcus aureus (staph).
"There's a huge, unmet clinical need for new approaches against staph skin infections because of declining antibiotic development and rising drug resistance," says Lloyd Miller, M.D., Ph.D., associate professor of dermatology at the Johns Hopkins University School of Medicine. "There are concerns that current antibiotics will not provide a durable solution to this public health threat."
Staph is a common bacterium and the most common cause of skin infections in people. Additionally, multidrug resistant strains, such as methicillin-resistant Staphylococcus aureus (MRSA), are causing severe skin infections in healthy people outside of hospitals. And once you've had an infection, the recurrence rate is 50 percent within six months. Staph can also spread from the skin and cause invasive and life-threatening infections such as sepsis, osteomyelitis and pneumonia. "This is particularly concerning for people with diabetes and those with compromised immune systems," says Miller.
"We couldn't have figured out what was happening without the expertise of our collaborators at U.C. Davis, who determined the genetic sequence of every single cell marker on the cells in the lymph nodes," says Miller.
That genetic sequencing data revealed that specific cells substantially multiplied after the initial infection, then moved to the infection site and provided protection against the second infection. These so-called gamma delta T cells account for less than 1 percent of all the cells in the lymph node prior to infection. After infection, they accounted for more than 20 percent.
"Our lab specializes in developing novel genetic methodologies to study T cell repertoires, but we had never applied this technology to study how the immune system responds to an infection," says Emanual Maverakis, M.D., associate professor of dermatology at the University of California, Davis School of Medicine. "We're thrilled to have played a role solving an important question that Dr. Miller had been working on for several years. This is just the first in a series of collaborative projects with the Miller lab that we have in store for the future."
Since this work was performed in mice, the team wanted to see if its findings were applicable to people. Working with collaborators from the National Institute of Allergy and Infectious Diseases at the National Institutes of Health, the researchers tested blood from healthy individuals and people with a rare immune disorder that makes them highly susceptible to staph skin infections.
According to Miller, half of people with the disorder die by age 10, but if they survive to adulthood they somehow overcome their susceptibility to staph infections. In blood samples from these patients, the researchers found an increase in the percentage of gamma delta T cells, similar to what they observed in mice, which remained stable over years.
"This was a totally unexpected result," says Miller. "We are excited to learn more about these cells and how they confer this long-lasting protective immunity."
Miller hopes these new findings and especially gamma delta T cells may be targeted for developing new therapies or even a vaccine against staph skin infections. This, he says, could alleviate the burden of staph skin infections, prevent invasive complications and reduce health care costs.
Miller receives grant support from MedImmune, Regeneron, Moderna Therapeutics and Pfizer, which are developing therapeutics and vaccines against staph and other pathogens. This work was supported by grant numbers R01AR069502 and R21AI126896 to Miller and 1DP2OD008752 to Maverakis, by the Division of Intramural Research of the National Institute of Allergy and Infectious Diseases at the National Institutes of Health, and an early career award from the Burroughs Wellcome Fund.