Every protein made in the cell has a specific destination and function, similar to passengers on an urban transit system.
Channels in cell membranes help direct these proteins to their appropriate target. Researchers at Boston University School of Medicine (BUSM) and their colleagues have now captured images of these channels as they open to allow proteins to pass through a membrane, while the proteins are being made. These findings are published as a Letter in Nature (Park, E. et al. 2013).
Christopher W. Akey, PhD, professor of physiology and biophysics at BUSM is a co-senior author of the Letter. In addition, the collaborating institutions include Harvard Medical School (HMS), Baylor College of Medicine (BCM) and Georgia Institute of Technology (GT).
In this study, researchers used samples made in E. coli bacteria to determine the structure of the highly conserved SecY channel. Using an electron microscope and computer analysis, researchers were able to capture images of the SecY channel opening when a nascent protein enters the central pore. In particular, the channel undergoes large movements that enlarge the central pore as a first step in allowing the nascent protein to cross the cell membrane and eventually travel to its destination.
"Similar to train cars that transport passengers through a tunnel, SecY/Sec61 channels help nascent proteins move across the cell membrane to reach their target in the body, and this study provides important insight about the function of these channels," said Akey.