Cytoglobin can maintain a minimal, but continuous supply of intracellular oxygen during ischemic and anoxic conditions although it is a temporary oxygen reservoir.
A research team from China Medical University was the first to use a plasmid carrying green fluorescent protein as the carrier to construct recombinant plasmids expressing cytoglobin by genetic engineering methods. Then, the recombinant plasmid was transfected into SH-SY5Y cells. Xiuling Yu and colleagues found that overexpression of cytoglobin could protect SH-SY5Y cells against cobalt chloride-induced hypoxia. The researchers investigate the neuroprotective ways from the perspective of in vitro genetic engineering, thereby providing reliable evidence for gene therapy of hypoxic-ischemic neurological diseases. The relevant findings were published in the Neural Regeneration Research (Vol. 8, No. 23, 2013).