Classical methods to detect schistosomiasis infections fail in individuals who release low numbers of schistosome eggs in their feces. However, the Helmintex method, which uses a magnetic field to isolate eggs, is more sensitive for these low intensity schistosomiasis infections, shows study published in PLOS Neglected Tropical Diseases.
Schistosomiasis is a common infection that affects over 290 million individuals, especially in Sub-Saharan Africa, Asia and South America. Classical diagnostic methods, including the Kato-Katz (KK) fecal smear recommended by the World Health Organziation, are not accurate in situations where low egg burden is present in stool. Alternative methods, including a point-of-care immunodiagnostic for schistosome cathodic circulating antigen in urine (POC-CCA) and the Helmintex (HTX) method, have been proposed as substitutes.
In the new work, Carlos Graeff-Teixeira of the Pontificia Universidade Catolica de Rio Grande de Sul, and colleagues compared the KK, POC-CCA and HTX methods in a population-based study. 461 children and adults from Candeal, Brazil provided stool, urine, and blood samples for the comparison; an additional 220 submitted at least one specimen type.
"The HTX method is very sensitive and should be used as a reference method for diagnosing intestinal schistosomiasis and for comparative evaluation of other tests," the researchers say. "The HTX method should also be considered for use in the monitoring and certification of transmission interruption."