Highlights
- A mutant protein has been isolated from sheep suffering from a prion disease called scrapie.
- These mutant prions multiplied in lab settings outside of the animal, but did not cause infection when transferred to other animals.
- This may be because the mutant prion lacks a stretch of amino acids that may be essential for infection in other animals.
Prions are cellular proteins that have become mis-folded and can trigger other proteins to mis-fold, aggregate into dense protein structures, and cause brain damage.
Prions are responsible for several infectious fatal neurodegenerative disorders, including Creutzfeldt-Jakob disease in humans and "mad cow disease" in cattle.
Previous research has shown that, under certain conditions, prion strains can mutate--change their conformation.
In the new study, Vanni's team induced the emergence of a prion mutant while working with highly diluted natural prions obtained from sheep suffering from a prion disease called scrapie.
However, when the researchers infected another animal, bank voles with the mutant protein, none of the voles showed signs of disease.
The mutant prion's inability to infect bank voles may be due to its missing a stretch of amino acids that is present in the un-mutated form.
The researchers hypothesize that this region-the stretch of amino acids- is essential for animal infection, but not for in vitro multiplication.
"By investigating the in vitro evolution of prion populations derived from natural scrapie, we found that the cloud of conformational variants also includes 'defective' variants which are unable to self-sustain in vivo.” the autors explain.
The ability of the mutant prions to self-replicate in vitro but not in vivo will hopefully contribute to find out the molecular mechanisms allowing misfolded prion protein conformers to become deadly prions.
Future studies could build on this finding to pinpoint key prion features that affect infectivity, improving understanding of these deadly proteins.
Source-Medindia