Mesenchymal stem cells
(MSCs) are found in human adult tissues such as synovial tissues, bone marrow,
and adipose tissues. They are derived from mesoderm and are differentiated into
cartilage, bone, adipose tissues and muscles. Many scientists believe that adult
stem cells are quite useful in transplanting into the damaged tissues.
regeneration requires enhancement of chondrogenic potential, better handling
operation and better MSCs adhesion efficacy. The application of aggregate
mesenchymal stem cells (MSCs) obtained from synovium is a hopeful option for
Shiro Suzuki and
colleagues investigated the usefulness and properties of aggregates of synovial
mesenchymal stem cells as a potent source of cartilage regeneration.
For the purpose of
study, hanging drop technique was used to aggregate both human and rabbit
synovial mesenchymal stem cells.
Both in vitro and in
vivo chondrogenic potential of synovial mesenchymal stem cells aggregates were
In rabbits, in vivo
studies showed mesenchymal stem cells
aggregates sticking by surface tension on the osteochondral defects. The
in vitro studies revealed synthesis of high amount of pellets of cartilage
matrix obtained from mesenchymal stem cells aggregates in comparison to pellets
of mesenchymal stem cells cultured in monolayer.
It was noticed that
transplantation of mesenchymal stem cells aggregates at low density resulted in
successful cartilage regeneration as compared to transplantation at high
density because at high density loss of nutrient and cell death of mesenchymal
stem cells occurred.
It was finally
accepted that mesenchymal stem cells aggregate derived from synovium were a
potential source of cartilage regeneration owing to their easy preparation,
able attachment and high chondrogenic potential.
Properties and usefulness of aggregates of
synovial mesenchymal stem cells as a source for cartilage regeneration; Shiro
Suzuki et al; Arthritis Research and Therapy 2012