IVF Pregnancy Success Rates Depend On Uterine Microbiota

Highlights

The uterine cavity has been considered sterile.
The uterine microbiota play an important on the success rates of IVF pregnancies.
Colonization of the uterine cavity with non Lactobacillus-dominated microbiota affect the success of in vitro fertilization, pregnancy rates, and live births.

Bacteria in the uterine cavity, known as endometrial microbiota, play an important role in determining whether women would get pregnant via in vitro fertilization (IVF).

This new study is published in the American Journal of Obstetrics and Gynecology.

In Vitro Fertilization (IVF) - Introduction To Infertility and Reproduction
Encyclopedia section of medindia gives a brief introduction to infertility and reproduction

‘Diagnostic tests to assess the microbial composition of the uterine cavity may be useful in determining the success rates of in vitro fertilization.’

Tweet it Now

"The uterine cavity has been considered sterile," explained lead investigator Carlos Simón, MD, PhD, of Igenomix Spain, and the Department of Obstetrics and Gynecology of the University of Valencia, Spain.

Study

The researchers sought to test the existence of endometrial microbiota which differ from that of the vagina. They also assessed the hormonal regulation and analyzed the impact of the endometrial microbial community on the reproductive outcome in patients undergoing IVF.

Culture Media Used in IVF Affects Birth Outcome
The environment in early life, from foetus to the first two years of life, can have a significant effect on long-term health.

To identify the existence of an endometrial microbiota- Investigators recruited 13 fertile women within the same menstrual cycle, who were in their pre-receptive and receptive phases. They then assessed paired samples of endometrial and vaginal fluid obtained from them.
To investigate the hormonal regulation of the endometrial microbiota- Investigators recruited 22 fertile women within the same cycle and collected endometrial fluid at pre-receptive and receptive phases.
To assess the impact of altered endometrial microbiota in endometrial fluid, on the reproductive outcome- Investigators recruited 35 infertile patients undergoing IVF and assessed their implantation, ongoing pregnancy, and live birth rates.

Findings

Difference in bacterial communities between the endometrial and vaginal sites in some women were detected, on examination of the sites.

IVF Surrogacy Can Benefit Woman With Underdeveloped Vagina
A 29-year-old female having a rare medical problem -- in which patients have an underdeveloped vagina and uterus -- was able to conceive through IVF surrogacy.

The microbiota in the endometrial fluid was classified as either Lactobacillus-dominated or non-Lactobacillus-dominated microbiota.

Women with a non-Lactobacillus-dominated microbiota and a receptive endometrium had a significantly lower rate of implantation, pregnancy, and live birth compared to patients with a Lactobacillus-dominated microbiota.

IVF �Add-On� Treatments Do More Harm Than Good
Majority of the add-on fertility treatments offered by the fertility clinics do not improve chances of conception.

The role of steroid hormones in determining the composition of the microbial community in the endometrium was not evident.

Future Scope

Roberto Romero, MD, DMedSci, Chief of the Perinatology Research Branch of NICHD/NIH and Editor-in-Chief for Obstetrics of the American Journal of Obstetrics and Gynecology, commented that "the endometrial cavity is not sterile and normally contains bacteria; however, whether bacteria within the cavity play a role in successful reproduction has been unknown to this point. The pioneering work of the team of Professor Simón now shows, for the first time, that colonization of the uterine cavity with some bacteria (non Lactobacillus-dominated) affects the success of in vitro fertilization, pregnancy rates, and live births.”

The success rates of IVF can be tested by using diagnostic tests to examine the microbial composition of the uterine cavity.

Similarly conducting targeted treatment using antimicrobial agents or probiotics may be useful in improving reproductive success.

The researchers conclude that successful reproduction may depend on host-microbial relationship in the endometrial cavity, which was unknown before this study.

Reference

Carlos Simon et al . Evidence that the endometrial microbiota has an effect on implantation success or failure. American Journal of Obstetrics and Gynecology; (2016) DOI: http://dx.doi.org/10.1016/j.ajog.2016.09.075

Source-Medindia