- New HIV test has been developed that can detect HIV antibodies in a saliva sample.
- Preliminary evaluation indicates that it is sensitive in detecting HIV positive cases.
- Further evaluation of the test is required before it can be introduced for routine use.
New test that can screen patients reliably for HIV using a saliva sample has been developed. Scientists published the details in the Proceedings of the National Academy of Sciences.
Early detection of HIV is necessary so that treatment can be initiated early and precautions can be taken to prevent the spread to others. Blood tests are currently the most sensitive in detecting HIV. These, however, need proper precautions and disposal of needles especially if the patient tests positive. It may not be possible to screen a large number of individuals through a blood test.
The scientists developed a test for HIV that can be done on a saliva sample instead. They realized that obtaining a saliva sample is much simpler than drawing blood for screening large populations for HIV. People may be more willing to give a saliva sample rather than a blood sample. Since the salivary samples do not contain the virus and are non-infective, this approach will also be safer for health care professionals. However, as compared to blood, antibodies to HIV do not accumulate in the saliva early enough or in large amounts. Thus, individuals may be negative even if they harbor the HIV virus in their bodies.
Scientists tested this new approach, called Antibody Detection by Agglutination-PCR (ADAP), in 44 individuals, 22 of whom were HIV positive detected through previous tests, while another 22 were HIV negative. They found that the test:
- Accurately detected the presence of HIV in all the HIV positive individuals.
- Did not produce any false positive results - None of the individuals who were negative for HIV wrongly tested positive by this test.
- Did not detect HIV before blood tests though it is 1,000-10,000 times more analytically sensitive than clinical enzyme-linked immunoassays (EIAs) and may detect the presence of HIV earlier than other saliva tests.
About HIV TestingThe Centers for Disease Control and Prevention (CDC) of the United States recommends testing for HIV at least once in all individuals between the ages of 13 and 64 years. This is particularly important in the following individuals or conditions:
- Homosexual males
- Having sex with HIV positive partners, with multiple partners or with someone whose HIV status is not known
- Drug addicts that share injection drugs
- Commercial sex workers
- Diagnosed earlier with a sexually transmitted disease, hepatitis or tuberculosis
- Having sex with a person positive for any of the above
- Pregnant women
Tests commonly used to detect HIV include:
- Nucleic acid tests (NAT): These directly test for the presence of the virus, and sometimes the amount of virus in the sample. They are expensive and therefore not used routinely for screening.
- Antigen/antibody tests: These are the most commonly done tests. A fourth-generation EIA assay that detects both antibodies and the p24 antigen is currently available and is a preferred test.
- Antibody tests: These include the ELISA or enzyme immunoassay (EIA), particle agglutination, and chemiluminescent immunoassay (CIA)
- Tsai C et al. Antibody detection by agglutination-PCR (ADAP) enables early diagnosis of HIV infection by oral fluid analysis. Proceedings of the National Academy of Sciences. Doi: 10.1073/pnas.1711004115
- HIV Basics Testing - (https://www.cdc.gov/hiv/basics/testing.html)
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Dr. Simi Paknikar. 2021. New Sensitive HIV Test on Saliva Sample. Medindia, viewed Sep 26, 2022, https://www.medindia.net/news/healthinfocus/new-sensitive-hiv-test-on-saliva-sample-176446-1.htm.