- Pluripotent or
embryonic stem cells (PSC) can differentiate between all types of tissues in
- PSCs are
therefore useful for research in the field of regenerative and reparative
- Identification of
cell specific markers of immature PSCs will simplify their cultivation and use
for research purposes
The discovery of cell surface markers for
the earliest embryonic stem cells by scientists at the Karolinska
Institutet has given a huge impetus to stem cell and early embryonic
of the Study
‘Identification of cell specific surface markers to differentiate immature and mature embryonic stem cells gives a huge boost to stem cell research, and the field of regenerative medicine.’
Though the embryonic stem cells
that are currently
used in research are isolated from the pre-implantation stage of the embryo, it
is highly likely that these stem cells have assumed a post-implantation state
with consequent limitations.
Na´ve or pre-implantation pluripotent or embryonic stem cells (PSCs) can be obtained from primed
post-implantation PSC resetting, but current
methods do not allow isolation of unmodified na´ve cells.
Ability to identify and isolate na´ve
stem cells which are still not primed is important in the field of stem cell and
research in early embryonic development.
Na´ve cells are easier to cultivate and
manipulate for research purposes and na´ve
PSCs permit generation of tissue types that are difficult with primed PSCs.
of the Research
- Using a set of antibody based screens, the research team was able to
determine cell specific markers for the na´ve and primed pluripotent stem
- By validating a set of antibodies in
culture lines of multiple na´ve and primed PSCs, the scientists established a set of proteins that
were expressed specifically on the pre-implantation but not the
post-implantation embryo, confirming the specificity of the
- The team created an antibody panel
targeting multiple cell surface antigens and showed that the antibody
panel could differentiate between na´ve and primed PSCs,
follow the dynamics of na´ve-primed interconversion, and identify na´ve
PSCs from a mixed cell population.
- The antibody panel developed can be used in flow cytometry, a
commonly used technique for sorting a heterogenous population of cells.
"We've not had cell surface markers
for the different stem cell states before, which has made it hard to study
them," says Fredrik Lanner, Assistant Professor at Karolinska Institutet's
Department of Clinical Science, Intervention and Technology. "We now have
a simple tool for identifying and sorting the cells, which benefits future stem
cell research and basic research on early embryonic development."
Benefits of The Research
Application of PSCs in Medicine
- Na´ve PSCs are capable of
differentiating into all tissues of the body
and assume importance in the field of reparative and regenerative medicine
- Some tissue types that are difficult
to obtain from primed PSC can be obtained from na´ve PSC
- The molecular events leading to
na´ve PSC resetting can be better delineated
- Cultivation and manipulation of
na´ve PSC would become easier
- Isolating na´ve PSC also paves the
way for early embryonic and infertility research
- Because of their unique properties, pluripotent
stem cellsá can be used to generate
any tissue type a person may need to counter a variety of diseases ranging
from diabetes, Alzheimer's disease, spinal cord injury, muscular dystrophy and leukemia to name a few.
- Pluripotent cells can be engineered
to provide a perfect HLA match for any patient without the need for tissue
typing and incidental rejection issues.
- PSCs find innumerable applications
in research and analysis of disease pathogenesis and evolution and provide
the opportunity to correct it in the earliest stages of development.
In conclusion, the delineation of na´ve
PSC has opened up a window of opportunities and techniques that are waiting to
be tapped into during
the course of future research.
- Stem Cell Basics III. - (https://stemcells.nih.gov/info/basics/3.htm)
- Why are Pluripotent Stem Cells Important? - (http://stemcell.childrenshospital.org/about-stem-cells/pluripotent-stem-cells-101/why-are-pluripotent-stem-cells-important/)