Obesity and type 2 diabetes can be cured using gene therapy in mice, reports a new study. The findings of the study are published in the journal EMBO Molecular Medicine. A single administration of an adeno-associated viral vector (AAV) carrying the FGF21 (Fibroblast Growth Factor 21) gene, resulted in genetic manipulation of the liver, adipose tissue or skeletal muscle to continuously produce the FGF21 protein. This protein is a hormone secreted naturally by several organs that acts on many tissues for the maintenance of correct energy metabolism. By inducing FGF21 production through gene therapy, the animal lost weight and decreased insulin resistance, which causes the development of type 2 diabetes.
‘Gene therapy promotes healthy aging and prevents age-associated weight gain and insulin resistance.’
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The therapy has been tested successfully in two different mouse models of obesity, induced either by diet or genetic mutations. Besides, the authors observed that when administered to healthy mice, the gene therapy promoted healthy aging and prevented age-associated weight gain and insulin resistance.After treatment with AAV-FGF21, mice lost weight and reduced fat accumulation and inflammation in adipose tissue; fat content (steatosis), inflammation and fibrosis of the liver were also reversed; this led to an increase in insulin sensitivity and healthy aging, without any adverse side effects.
The results have been reproduced after genetic manipulation of three different tissues (liver, adipose tissue or skeletal muscle) to produce the FGF21 protein. "This gives great flexibility to the therapy, since it allows to select each time the most appropriate tissue, and in case some complication prevents manipulating any of the tissues, it can be applied to any of the others. When a tissue produces FGF21 protein and secretes it into the bloodstream, it will be distributed throughout the body", states the director of the study Dr. Fatima Bosch.
The authors highlight the importance of these results, since "the prevalence of type 2 diabetes and obesity is growing at alarming rates around the world", explains the UAB researcher and co-author of the study Claudia Jambrina. Obesity also increases the risk of mortality and represents an important risk factor for cardiovascular and immune diseases, hypertension, arthritis, neurodegenerative disorders and some types of cancer.
"This is the first time that long-term reversion of obesity and insulin resistance have been achieved upon a one-time administration of gene therapy, in an animal model that resembles obesity and type 2 diabetes in humans", explained the first author of the paper and UAB researcher Veronica Jimenez. "The results demonstrate that it is a safe and effective therapy."
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The native FGF21 protein has a short half-life when administered using conventional procedures. For this reason, the pharmaceutical industry has developed FGF21 analogs/mimetics and has already conducted clinical trials. FGF21 analogs/mimetics, however, require the periodic administration to mediate clinical benefits but may raise immunological issues associated to the administration of exogenous proteins. The gene therapy vectors used by Dr. Bosch's team, however, induce the mice to produce for many years the same FGF21 hormone naturally produced by the body, after a single administration and without any adverse effects.
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The study was conducted by researchers from the Centre for Animal Biology and Gene Therapy (CBATEG), the Departments of Biochemistry and Molecular Biology and of Animal Health and Anatomy of the Universitat Autnoma de Barcelona, and from the CIBER de Diabetes y Enfermedades Metablicas Asociadas (CIBERDEM).
Source-Eurekalert