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Cryopreservation of Induced Pluripotent Stem Cells Improved the Most by One Product: Research

by Rukmani Krishna on September 27, 2012 at 12:27 AM
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 Cryopreservation of Induced Pluripotent Stem Cells Improved the Most by One Product: Research

A team of researchers from Japan compared 12 kinds of commercially prepared and readily available cryopreservation solutions and found that "Cell Banker 3" out-performed the other 11 solutions by allowing iPS cells to be preserved for a year at 󈞼 degrees C in an undifferentiated state, in a study to determine the best cryopreservation (freezing) solution to maintain induced pluripotent stem (iPS) cells. The study is published in a recent special issue of Cell Medicine [3(1)], now freely available on-line at http://www.ingentaconnect.com/content/cog/cm.

"iPS cells are a promising alternative to embryonic stem cells and can be used in place of bone marrow cells, stromal cells and adipose tissue-derived stem cells," said study co-author Hirofumi Noguchi, MD, PhD, Department of Gastroenterological Surgery, Transplant and Surgical Oncology at the Okayama University Graduate School of Medicine.

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"However, the viability of human iPS cells, like embryonic stem cells, decreases significantly during cryopreservation. A wide variety of cryopreservation solutions have been used, however many are toxic or ineffective for use in extended cryopreservation."The researchers concluded that Cell Banker 3 showed the highest cell viability and proliferation of all the solutions examined and can be widely used as it does not require any special skills for use.

Source: Eurekalert
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